antibody

GBL (Target of rapamycin complex subunit LST8,MLST8,GBL, LST8) discontinued

030985

200 ul

monoclonal

mouse

nonconjugated

211CT7.1.1

GBL (Target of rapamycin complex subunit LST8,MLST8,GBL, LST8) discontinued

mouse

Mab

Antibodies

200 ul

211CT7.1.1

IgG1,k

Subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with FRAP1 and enhances its kinase activity. In nutrient-poor conditions, stabilizes the FRAP1-RPTOR interaction and favors RPTOR-mediated inhibition of FRAP1 activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Applications: Suitable for use in Western Blot, ELISA. Recommended Dilution: ELISA: 1:1000 Western Blot: 1:15~60 Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Mo

Target of rapamycin complex subunit LST8,MLST8,GBL, LST8

GBL Mab is generated from mouse immunized with GBL recombinant protein.

Mouse

Purified by Protein G affinity chromatography.

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

As reported

Product Type: Mab Isotype: IgG1,k Clone No: 211CT7.1.1 Host: mouse Source: mouse Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. Purity: Purified by Protein G affinity chromatography. Immunogen: GBL Mab is generated from mouse immunized with GBL recombinant protein. Specificity: Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

-20°C

Ali, S.M., et al. J. Biol. Chem. 280(20):19445-19448(2005) Inoki, K., et al. Microbiol. Mol. Biol. Rev. 69(1):79-100(2005) Sarbassov, D.D., et al. Science 307(5712):1098-1101(2005) Jacinto, E., et al. Nat. Cell Biol. 6(11):1122-1128(2004) Kim, D.H., et al. Mol. Cell 11(4):895-904(2003)