This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
Chk 1 (Checkpoint Kinase 1, CHEK1, CHK1, Serine/threonine-protein Kinase Chk1) (Biotin) discontinued
catalog :
030874-Biotin
quantity :
200 ul
clonality :
monoclonal
host :
mouse
conjugate :
biotin
clone name :
2G1D5

The same clone is also sold as:
reactivity :
human
product information
Catalog Number :
030874-Biotin
Product wo Prefix :
Chk 1 (Checkpoint Kinase 1, CHEK1, CHK1, Serine/threonine-protein Kinase Chk1) (Biotin) discontinued
Host :
mouse
Product Type :
Mab
Conjugate :
Biotin
Category :
Antibodies
Size1 :
200 ul
Clone # USB :
2G1D5
Isotype :
IgM
Desc1 :
Chk1, one of the downstream protein kinases of ATM/ATR, plays an important role in DNA damage checkpoint, embryonic development and tumor suppression. The activation of Chk1 in response to replication blocks and certain forms of genotoxic stress involves phosphorylation of serines 317 and 345. Activated Chk1 can inactivate cdc25c via phosphorylation at serine 216, blocking the activation of cdc2 and transition into M-phase. Chk1 can also phosphorylate p53 at serine 20 in vitro. Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2-DNA damage checkpoint by phosphorylating CDC25C in response to DNA damage. CDC25A, CDC25B and CDC25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating CDC2 p34 on regulatory tyrosine residues. Phosphorylation of CDC25C by Chk1 and 2 inhibits its phosphatase activity, thereby blocking mitosis. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1. Applications: Suitable for use in Western Blot, ELISA. Recommended Dilution: ELISA: 1:10,000 Western Blot: 1:1,000~4,000 Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Applications are based on unconjugated antibody.
Calc Crossreactivity :
Hu Mo
Immunogen :
Ni-NTA purified recombinant protein CHK1 expressed in E. Coli strain M15.
Specificity :
Human, mouse
Purity :
Purified by Protein G affinity chromatography.
Form :
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Concentration :
As reported
Desc2 :
Product Type: Mab Isotype: IgM Clone No: 2G1D5 Host: mouse Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin. Purity: Purified by Protein G affinity chromatography. Immunogen: Ni-NTA purified recombinant protein CHK1 expressed in E. Coli strain M15. Specificity: Human, mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E WB
Storage Temperature :
-20°C
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/19/030874-Biotin_1.jpg
company information
US Biological
4 Technology Way
Salem, MA01970
service@usbio.net
https://www.usbio.net
800-520-3011
headquarters: USA
United States Biological is a primary brand for the Life Science Industry. We are committed to reducing the cost of research with value, integrity and a truly personal buying experience.