antibody

ATG12 (ATG12, APG12, APG12L, Ubiquitin-like protein ATG12, Autophagy-related protein 12) (Biotin)

030788-Biotin

200 ul

monoclonal

mouse

biotin

43CT73.3.5.5.4

The same clone is also sold as:

• Invitrogen: MA5-37467
• Abnova: MAB12281

ATG12 (ATG12, APG12, APG12L, Ubiquitin-like protein ATG12, Autophagy-related protein 12) (Biotin)

mouse

Mab

Biotin

Antibodies

200 ul

43CT73.3.5.5.4

IgG2b

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]). Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended Dilution: ELISA : 1:10000 Western Blot: 1:200~2000 Immunohistochemistry: 1:50~200 Immunofluorescenc: 1:10~50 Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

Hu

ATG12, APG12, APG12L, Ubiquitin-like protein ATG12, Autophagy-related protein 12

APG12L recombinant protein.

Human

Purified by Protein G affinity chromatography.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

As reported

Product Type: Mab Isotype: IgG2b Clone No: 43CT73.3.5.5.4 Host: mouse Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin. Purity: Purified by Protein G affinity chromatography. Immunogen: APG12L recombinant protein. Specificity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IF IHC WB

-20°C

Control of basal autophagy by calpain1 mediated cleavage of ATG5. Xia HG, et al. Autophagy, 2010 Jan. PMID 19901552. Frameshift mutations of autophagy-related genes ATG2B, ATG5, ATG9B and ATG12 in gastric and colorectal cancers with microsatellite instability. Kang MR, et al. J Pathol, 2009 Apr. PMID 19197948. Regulation of autophagy by the p300 acetyltransferase. Lee IH, et al. J Biol Chem, 2009 Mar 6. PMID 19124466. Mitochondrial DNA deletions and chloramphenicol treatment stimulate the autophagic transcript ATG12. Prigione A, et al. Autophagy, 2007 Jul-Aug. PMID 17457038. Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.

https://usbio-images.r.worldssl.net/prodimages/44/030788-Biotin_1.jpg

Atg12 antibody tested in HEK cells. Fixed in PFA permeablilized with 0.2% Saponin. Blocked and antibodies in 10% goat serum. Secondary was mouse 555. The right is in full medium (FM) and the left with EBSS (no leupeptin)(Provided by Nicole McKnight & Sharon Tooze).