antibody

ATG5 (Autophagy Protein 5, APG5-like, Apoptosis-specific Protein, APG5L, ASP) (Biotin)

030779-Biotin

200 ul

monoclonal

mouse

biotin

22CT1.11.4.4

ATG5 (Autophagy Protein 5, APG5-like, Apoptosis-specific Protein, APG5L, ASP) (Biotin)

mouse

Mab

Biotin

Antibodies

200 ul

22CT1.11.4.4

IgG2a,k

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. APG5 conjugates to APG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. It localise to the cytoplasm and it co-localise with nonmuscle actin. APG5 is ubiquitously expressed. Its expression is dramatically increased in apoptotic cells. Conjugation to APG12 is essential for autophage but it is not required for association with isolation membrane. Applications: Suitable for use in Western Blot, ELISA. Recommended Dilution: ELISA: 1:1000 Western Blot: 1:1000 Immunohistochemistry: 1:50~100 Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

Hu

Purified recombinant APG5 fusion protein.

Human

Purified by Protein G affinity chromatography.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

As reported

Product Type: Mab Isotype: IgG2a,k Clone No: 22CT1.11.4.4 Host: mouse Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin. Purity: Purified by Protein G affinity chromatography. Immunogen: Purified recombinant APG5 fusion protein. Specificity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IHC WB

-20°C

Age at onset in Huntington's disease is modified by the autophagy pathway: implication of the V471A polymorphism in Atg7. Metzger S, et al. Hum Genet, 2010 Oct. PMID 20697744. Autophagy protein ATG5 interacts transiently with the hepatitis C virus RNA polymerase (NS5B) early during infection. Guévin C, et al. Virology, 2010 Sep 15. PMID 20580051. The Bcl-2 homology domain 3 mimetic gossypol induces both Beclin 1-dependent and Beclin 1-independent cytoprotective autophagy in cancer cells. Gao P, et al. J Biol Chem, 2010 Aug 13. PMID 20529838. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614. Morphine induces Beclin 1- and ATG5-dependent autophagy in human neuroblastoma SH-SY5Y cells and in the rat hippocampus. Zhao L, et al. Autophagy, 2010 Apr. PMID 20190558.