Actin, alpha (ACTA1, ACTA, skeletal muscle, Alpha-actin-1) | US Biological 030740 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Actin, alpha (ACTA1, ACTA, skeletal muscle, Alpha-actin-1)

catalog :

030740

quantity :

200 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

337CT30.10.1

The same clone is also sold as:

Invitrogen: MA5-37536
Abnova: MAB12316

reactivity :

human

product information

Catalog Number :

030740

Product wo Prefix :

Actin, alpha (ACTA1, ACTA, skeletal muscle, Alpha-actin-1)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

200 ul

Clone # USB :

337CT30.10.1

Isotype :

IgG1,k

Desc1 :

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. Applications: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1:100-1:500 Immunohistochemistry (FFPE): 1:10-1:50 Flow Cytometry: 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Alternate Name :

ACTA1, ACTA, Actin, alpha skeletal muscle, Alpha-actin-1

Immunogen :

Recombinant protein corresponding to human Actin, alpha. Species Sequence Homology: bovine, chicken, mouse, procine, rabbit and rat

Specificity :

Recognizes human Actin, alpha.

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Concentration :

As Reported

Desc2 :

Product Type: Mab Isotype: IgG1,k Clone No: 337CT30.10.1 Host: mouse Source: human Concentration: As Reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by Protein G affinity chromatography. Immunogen: Recombinant protein corresponding to human Actin, alpha. Species Sequence Homology: bovine, chicken, mouse, procine, rabbit and rat Specificity: Recognizes human Actin, alpha. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IHC WB

Storage Temperature :

-20°C

Reference :

1. Kim, E.Y., et al. Am. J. Physiol. Renal Physiol. 299 (3), F594-F604 (2010) 2. Haigh, S.E., et al. Neuromuscul. Disord. 20(6):363-374(2010) 3. Yu, G., et al. J Clin Neurosci 17(6):766-769(2010) 4. Yu, C.H., et al. PLoS ONE 5 (7), E11878 (2010) 5. Licastro, F., et al. Curr. Pharm. Des. 16(7):783-788(2010)

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/12/030740_1.jpg

Picture4 Text :

Flow Cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram) using 030740. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.