Actin, alpha (ACTA1, ACTA, skeletal muscle, Alpha-actin-1) (APC) | US Biological 030740-APC product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Actin, alpha (ACTA1, ACTA, skeletal muscle, Alpha-actin-1) (APC)

catalog :

030740-APC

quantity :

200 ul

clonality :

monoclonal

host :

mouse

conjugate :

APC

clone name :

337CT30.10.1

The same clone is also sold as:

Abnova: MAB12316
Invitrogen: MA5-37536

reactivity :

human

product information

Catalog Number :

030740-APC

Product wo Prefix :

Actin, alpha (ACTA1, ACTA, skeletal muscle, Alpha-actin-1) (APC)

Host :

mouse

Product Type :

Mab

Conjugate :

APC

Category :

Antibodies

Size1 :

200 ul

Clone # USB :

337CT30.10.1

Isotype :

IgG1,k

Desc1 :

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. Applications: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry and FLISA. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Calc Crossreactivity :

Alternate Name :

ACTA1, ACTA, Actin, alpha skeletal muscle, Alpha-actin-1

Immunogen :

Recombinant protein corresponding to human Actin, alpha. Species Sequence Homology: bovine, chicken, mouse, procine, rabbit and rat

Specificity :

Recognizes human Actin, alpha.

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

Concentration :

As Reported

Desc2 :

Product Type: Mab Isotype: IgG1,k Clone No: 337CT30.10.1 Host: mouse Source: human Concentration: As Reported Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC). Purity: Purified by Protein G affinity chromatography. Immunogen: Recombinant protein corresponding to human Actin, alpha. Species Sequence Homology: bovine, chicken, mouse, procine, rabbit and rat Specificity: Recognizes human Actin, alpha. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC FL IHC WB

Storage Temperature :

4°C Do Not Freeze

Reference :

1. Kim, E.Y., et al. Am. J. Physiol. Renal Physiol. 299 (3), F594-F604 (2010) 2. Haigh, S.E., et al. Neuromuscul. Disord. 20(6):363-374(2010) 3. Yu, G., et al. J Clin Neurosci 17(6):766-769(2010) 4. Yu, C.H., et al. PLoS ONE 5 (7), E11878 (2010) 5. Licastro, F., et al. Curr. Pharm. Des. 16(7):783-788(2010)

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/37/030740-APC_1.jpg

Picture4 Text :

Flow Cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram) using 030740. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.