SHH (Sonic hedgehog) | US Biological 030622 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

SHH (Sonic hedgehog)

catalog :

030622

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

13B700

reactivity :

human

product information

Catalog Number :

030622

Product wo Prefix :

SHH (Sonic hedgehog)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

13B700

Isotype :

IgG1

Desc1 :

This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly. Applications: Western Bloting: 1:500-1:2000. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Alternate Name :

TPT; HHG1; HLP3; HPE3; SMMCI; TPTPS; MCOPCB5; SHH

Immunogen :

Purified recombinant fragment of human SHH expressed in E. coli.

Specificity :

Human

Purity :

Ascites

Form :

Ascites fluid, 0.03% sodium azide.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 13B700 Host: mouse Source: human Concentration: Not determined Form: Ascites fluid, 0.03% sodium azide. Purity: Ascites Immunogen: Purified recombinant fragment of human SHH expressed in E. coli. Specificity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

1. Cancer Lett. 2010 Jan 1;287(1):44-53. 2. Oncogene. 2009 Oct 8;28(40):3513-25. 3. J Biol Chem. 2009 Nov 20;284(47):32562-71.