PLK1 | US Biological 030550 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

PLK1

catalog :

030550

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

13B628

reactivity :

human

product information

Catalog Number :

030550

Product wo Prefix :

PLK1

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

13B628

Isotype :

IgG1

Desc1 :

PLK1 is critical for the initiation of centrosome maturation. Polo-like kinases (PLKs) are a family of four serine:threonine protein kinases that are critical regulators of cell cycle progression, mitosis, cytokinesis, and the DNA damage response. PLK1, -2 and -3 are ubiquitously expressed, whereas PLK4 is restricted to a few tissues including the testes and the thymus. The mRNA and protein expression of PLK1, -2 and -4 are coordinately regulated during cell cycle progression, but PLK3 levels are independent of the other three family members. Furthermore, PLK3 is a much more stable protein than PLK1, -2 or -4. PLK1 is the most well characterized member of this family and strongly promotes the progression of cells through mitosis. During the various stages of mitosis PLK1 localizes to the centrosomes, kinetochores and central spindle. PLKs are dysregulated in a variety of human cancers. PLK1 overexpression correlates with cellular proliferation and poor prognosis. PLK2 and PLK3 are involved in checkpoint-mediated cell cycle arrest to ensure genetic stability. Loss-of-function mutations in these enzymes can lead to oncogenic transformation. Applications: Western Blot: 1:500-1:2000 Immunocytochemistry: 1:50 ELISA: 1:10000 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Alternate Name :

PLK; STPK13

Immunogen :

AA: 331-508

Specificity :

Human

Purity :

Purified

Form :

Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer

Concentration :

~1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 13B628 Host: mouse Source: human Concentration: ~1mg/ml Form: Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer Purity: Purified Immunogen: AA: 331-508 Specificity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IC WB

Storage Temperature :

-20°C

Reference :

1.J Cell Biol. 2011 Dec 26;195(7):1093-101. 2.Eur J Cancer. 2011 Sep;47(14):2166-74.