PIK3CA (Phosphoinositide-3-kinase, catalytic, alpha polypeptide) | US Biological 030545 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

PIK3CA (Phosphoinositide-3-kinase, catalytic, alpha polypeptide)

catalog :

030545

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4F3

The same clone is also sold as:

Novus Biologicals: NBP2-37511-0.025ml
OriGene: AM06736PU-N
Abcam: ab126819

reactivity :

human

product information

Catalog Number :

030545

Product wo Prefix :

PIK3CA (Phosphoinositide-3-kinase, catalytic, alpha polypeptide)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

4F3

Isotype :

IgG1

Desc1 :

Phosphatidylinositol 3-kinase is composed of an 85kD regulatory subunit and a 110kD catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. Applications: Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:10,000. Immunocytochemistry/Immunofluorescence: 1:200-1:1,000. Flow cytometry: 1:200-1:400. Western Blot: 1:500-1:2,000. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Alternate Name :

PI3K; p110-alpha

Immunogen :

Purified recombinant fragment of human PIK3CA expressed in E. coli.

Specificity :

Recognizes human PIK3CA.

Purity :

Purified

Form :

Supplied as a liquid in PBS with 0.05% sodium azide.

Concentration :

~1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 4F3 Host: mouse Source: human Concentration: ~1mg/ml Form: Supplied as a liquid in PBS with 0.05% sodium azide. Purity: Purified. Immunogen: Purified recombinant fragment of human PIK3CA expressed in E. coli. Specificity: Recognizes human PIK3CA. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IC IF WB

Storage Temperature :

-20°C

Reference :

Cancer Res. 2009 Dec 1;69(23):8868-76. Diagn Mol Pathol. 2009 Dec;18(4):200-5.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/34/030545_1.jpg

Picture4 Text :

Western blot analysis using recombinant human PIK3CA (amino acids 881-1068) as sample. (Expected MW is 47.4kD).

Picture 5 File Name :

https://usbio-images.r.worldssl.net/prodimages/34/030545_5.jpg

Picture5 Text :

Western blot analysis using as samples: (1) HEK293 cell lysate and (2) PIK3CA (aa 881-1068)-hIgGFc-transtected cell lysate.