p63α (Tumor protein 63) | US Biological 030520 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

p63α (Tumor protein 63)

catalog :

030520

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

13B598

reactivity :

human

product information

Catalog Number :

030520

Product wo Prefix :

p63α (Tumor protein 63)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

13B598

Isotype :

IgG1

Desc1 :

The p63 gene is a homologue of the p53 tumor suppressor gene. Like p53, p63 contains a transactivation (TA) domain induce the transcription of target genes, a DNA binding domain, and an oligomerization domain (OD), used to form tetramers. In contrast to p53, the p63 gene encodes for at least six major isotypes. Three isotypes (TAp63α, TAp63β, and TAp63γ) contain the transactivating (TA) domain and are able to transactivate p53 report genes and induce apoptosis. In contrast, the other three isotypes (ΔNp63α, ΔNp63β, ΔNp63γ) are transcribed from an internal promoter localized within intron3, lack the TA domain, and act as dominant-negatives to suppress transactivation by both p53 and TAp63 isotypes. p63 is highly expressed in the basal cells of the epithelium significant for proper limb outgrowth and morphogenesis.4 In differentiating tissues, p63 is crucial for maintaining the stem cell identity of the basal cells, and is indispensable for correct development of the skin as well as the limb. p63-deficient mice lack all squamous epithelia and their derivatives, including hair, whiskers, teeth, as well as mammary, lacrimal, and salivary glands.Tissue specificity: Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues. Applications: Suitable for use in Western Blot, Immunohistochemsitry and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:2000. Immunohistochemistry: 1:200-1:1000. ELISA: Suggested dilution 1:10,000. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk Mo Rt

Alternate Name :

P51B; KET; p63α

Immunogen :

Synthesized peptide of human p63α.

Specificity :

Recognizes human p63α. Species Crossreactivity: mouse, rat, monkey.

Purity :

Ascites

Form :

Supplied as a liquid, 0.03% sodium azide.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 13B598 Host: mouse Source: human Concentration: Not determined Form: Supplied as a liquid, 0.03% sodium azide. Purity: Ascites Immunogen: Synthesized peptide of human p63α. Specificity: Recognizes human p63α. Species Crossreactivity: mouse, rat, monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC WB

Storage Temperature :

-20°C

Reference :

1. Cancer Res. 2008 Jul 1;68(13):5122-31. 2. Eur J Med Genet. 2008 Sep-Oct;51(5):497-500.