NF-κB p65 (Nuclear factor NF-kappa-B p65 subunit) | US Biological 030488 product information

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product summary

company name :

US Biological

product type :

antibody

product name :

NF-κB p65 (Nuclear factor NF-kappa-B p65 subunit)

catalog :

030488

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

13B566

reactivity :

human

product information

Catalog Number :

030488

Product wo Prefix :

NF-κB p65 (Nuclear factor NF-kappa-B p65 subunit)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

13B566

Isotype :

IgG1

Desc1 :

Transcription factors of the nuclear factor κ B (NF-κB)/Rel family is a ubiquitously expressed transcription factor that regulates many cytokine and Ig genes. It is involved in immune, inflammatory, viral, and acute phase responses. There are five family members in mammals: RelA (p65), c-Rel, RelB, NF-κB1 (p105/p50) and NF-κB2 (p100/p52). The most studied NF-κB complex consists of the p50 and p65 subunits, both containing a 300 amino acid region with homology to the Rel proto-oncogene product. The p50 subunit binds DNA, whereas the p65 subunit is responsible for the interaction of NF-κB with its inhibitor, IκB. In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to IκB. This complex prevents nuclear translocation and activity of NF-κB. In response to stimuli such as cytokines, LPS, and viral infections, IκB is phosphorylated at critical residues. This phosphorylation induces dissociation of the IκB/NF-κB complex, allowing the free heterodimeric NF-κB to form a heterotetramer that translocates to the nucleus. In the nucleus, it binds to the κB site within promoters and enhancers and functions as a transcriptional activator. Applications: Western Bloting: 1:500-1:2000. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mo

Alternate Name :

NFkappaB p65; p65; NFKB3; RELA

Immunogen :

Purified recombinant fragment of human NF-κB p65 expressed in E. coli.

Specificity :

Human, Mouse

Purity :

Ascites

Form :

Ascites fluid, 0.03% sodium azide.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 13B566 Host: mouse Source: human Concentration: Not determined Form: Ascites fluid, 0.03% sodium azide. Purity: Ascites Immunogen: Purified recombinant fragment of human NF-κB p65 expressed in E. coli. Specificity: Human, Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

1. Nature. 1997 Aug 7;388(6642):548-54. 2. Cell. 1998 Dec 11;95(6):749-58. 3. J Biol Chem. 2000 Jun 16;275(24):18180-7.