ELK1 | US Biological 030164 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

ELK1

catalog :

030164

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

13B242

reactivity :

human

product information

Catalog Number :

030164

Product wo Prefix :

ELK1

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

13B242

Isotype :

IgG1

Desc1 :

The transcription factor ELK1 is a family of member of ETS oncogene family and of the ternary complex factor (TCF) subfamily,which is located on chromosome Xp11.2 and stimulates transcription. binds to purine-rich DNA sequences. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Elk1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C terminus,It appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk1 is a good substrate for MAP kinase, the kinetics of Elk1phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk1 activation in vivo. More recent studies have shown that Elk1 is also a target of the Stress Activated Kinase SAPK/JNK. Phosphorylation of Elk1 has also been implicated in synaptic plasticity in the adult hippocampus. Applications: Western Bloting: 1:500-1:2000. Immunohistochemistry: 1:200-1:1000. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Alternate Name :

ELK1

Immunogen :

Purified recombinant fragment of ELK1 expressed in E. coli.

Specificity :

Human

Purity :

Ascites

Form :

Ascites fluid, 0.03% sodium azide.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 13B242 Host: mouse Source: human Concentration: Not determined Form: Ascites fluid, 0.03% sodium azide. Purity: Ascites Immunogen: Purified recombinant fragment of ELK1 expressed in E. coli. Specificity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC WB

Storage Temperature :

-20°C

Reference :

1. Rao,V.N., et al. 1989.Science.244 (4900):66-70. 2. Hsieh,Y.H., et al. 2006.Biochem. Biophys. Res. Commun. 339 (1): 217-225. 3. Gille,H., Strahl,T. and Shaw,P.E.1995. Curr. Biol. 5 (10): 1191-1200. 4. Gille,H., et al. 1995.EMBO J. 14 (5): 951-962.