EIF4E (Eukaryotic translation initiation factor 4E) | US Biological 030163 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

EIF4E (Eukaryotic translation initiation factor 4E)

catalog :

030163

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

13B241

reactivity :

human

product information

Catalog Number :

030163

Product wo Prefix :

EIF4E (Eukaryotic translation initiation factor 4E)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

13B241

Isotype :

IgG1

Desc1 :

eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk. Applications: Western Bloting: 1:500-1:2000. Immunohistochemistry: 1:200-1:1000. Immunofluorescence: 1:200-1:1000. Flow cytometry: 1:200-1:400. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Alternate Name :

CBP; EIF4F; EIF4E1; EIF4EL1; MGC111573; EIF4E

Immunogen :

Purified recombinant fragment of human EIF4E expressed in E. coli.

Specificity :

Human

Purity :

Ascites

Form :

Ascites fluid, 0.03% sodium azide.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 13B241 Host: mouse Source: human Concentration: Not determined Form: Ascites fluid, 0.03% sodium azide. Purity: Ascites Immunogen: Purified recombinant fragment of human EIF4E expressed in E. coli. Specificity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IHC WB

Storage Temperature :

-20°C

Reference :

1. Ann Surg Oncol. 2008 Nov;15(11):3207-15. 2. J Biol Chem. 2008 Sep 12;283(37):25227-37.