DKK3 (Dickkopf-related Protein 3, Dickkopf-3, Dkk-3, REIC, RIG, NQ258/PRO295)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

DKK3 (Dickkopf-related Protein 3, Dickkopf-3, Dkk-3, REIC, RIG, NQ258/PRO295)

catalog :

030149

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

13B227

reactivity :

human

product information

Catalog Number :

030149

Product wo Prefix :

DKK3 (Dickkopf-related Protein 3, Dickkopf-3, Dkk-3, REIC, RIG, NQ258/PRO295)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

13B227

Isotype :

IgG1

Desc1 :

This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50-55aa in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12aa. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, and spinal cord. In situ hybridization reveals highest expression in mouse brain, eye, and heart. Applications: Western Blot: 1:500-1:2000 Immunohistochemistry: 1:200-1:1000 Flow Cytometry: 1:200-1:400 ELISA: 1:10000 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Purified recombinant fragment of human DKK3 (AA: 91-350 ) expressed in E. coli.

Specificity :

Human

Purity :

Purified

Form :

Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer

Concentration :

As reported

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 13B227 Host: mouse Source: human Concentration: As reported Form: Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer Purity: Purified Immunogen: Purified recombinant fragment of human DKK3 (AA: 91-350 ) expressed in E. coli. Specificity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IHC WB

Storage Temperature :

-20°C

Reference :

1.Exp Dermatol. 2011 Mar;20(3):273-7. 2.Thromb Haemost. 2011 Jan;105(1):72-80.