Chk 1 (Checkpoint Kinase 1, CHEK1, CHK1, Serine/threonine-protein Kinase Chk1)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Chk 1 (Checkpoint Kinase 1, CHEK1, CHK1, Serine/threonine-protein Kinase Chk1)

catalog :

030095

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

13B173

reactivity :

human

product information

Catalog Number :

030095

Product wo Prefix :

Chk 1 (Checkpoint Kinase 1, CHEK1, CHK1, Serine/threonine-protein Kinase Chk1)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

13B173

Isotype :

IgG1

Desc1 :

CHK1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro. Applications: Western Bloting: 1:500-1:2000. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mo

Alternate Name :

CHEK1

Immunogen :

Purified recombinant fragment of CHK1 expressed in E. coli.

Specificity :

Human, Mouse

Purity :

Purified

Form :

Purified antibody in PBS, 0.03% sodium azide.

Concentration :

~1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 13B173 Host: mouse Source: human Concentration: ~1mg/ml Form: Purified antibody in PBS, 0.03% sodium azide. Purity: Purified Immunogen: Purified recombinant fragment of CHK1 expressed in E. coli. Specificity: Human, Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

1. EMBO J. 1998. 16: 545-554. 2. Science 1998. 282: 1893-1897.