Calreticulin (RO, CRT, SSA, cC1qR, FLJ26680, CALR) | US Biological 030017 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Calreticulin (RO, CRT, SSA, cC1qR, FLJ26680, CALR)

catalog :

030017

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

030017

Product wo Prefix :

Calreticulin (RO, CRT, SSA, cC1qR, FLJ26680, CALR)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG2a

Desc1 :

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes. Applications: Immunofluorescence: 1:200-1:1000. ELISA: 1:10000. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Synthetic peptide corresponding to amino acids E-E-E-D-V-P-G-Q-A-K-D-E-L-C of human Calreticulin, conjugated to KLH.

Specificity :

Human

Purity :

Serum

Form :

Serum

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG2a Host: rabbit Source: human Concentration: Not determined Form: Serum Purity: Serum Immunogen: Synthetic peptide corresponding to amino acids E-E-E-D-V-P-G-Q-A-K-D-E-L-C of human Calreticulin, conjugated to KLH. Specificity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IF

Storage Temperature :

-20°C

Reference :

1. J Biol Chem. 2006 May 5;281(18):12841-8. Epub 2006 Mar 9. 2. Biochim Biophys Acta. 2006 May;1760(5):745-53. Epub 2006 Feb 28. 3. Oncol Rep. 2007 May;17(5):1101-7.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/11/030017_1.jpg