BTK (Tyrosine-protein Kinase BTK, Agammaglobulinaemia Tyrosine Kinase, ATK, B Cell Progenitor Kinase, BPK, Bruton Tyrosine Kinase, AGMX1, ATK, BPK)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

BTK (Tyrosine-protein Kinase BTK, Agammaglobulinaemia Tyrosine Kinase, ATK, B Cell Progenitor Kinase, BPK, Bruton Tyrosine Kinase, AGMX1, ATK, BPK)

catalog :

030007

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

13B85

reactivity :

human

product information

Catalog Number :

030007

Product wo Prefix :

BTK (Tyrosine-protein Kinase BTK, Agammaglobulinaemia Tyrosine Kinase, ATK, B Cell Progenitor Kinase, BPK, Bruton Tyrosine Kinase, AGMX1, ATK, BPK)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

13B85

Isotype :

IgG1

Desc1 :

Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases.All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini.BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation. Applications: Western Bloting: 1:500-1:2000. Immunohistochemistry: 1:200-1:1000. Immunofluorescence: 1:200-1:1000. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk

Alternate Name :

BTK

Immunogen :

Purified recombinant fragment of BTK expressed in E. coli.

Specificity :

Human, Monkey

Purity :

Ascites

Form :

Ascites fluid, 0.03% sodium azide.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 13B85 Host: mouse Source: human Concentration: Not determined Form: Ascites fluid, 0.03% sodium azide. Purity: Ascites Immunogen: Purified recombinant fragment of BTK expressed in E. coli. Specificity: Human, Monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC WB

Storage Temperature :

-20°C

Reference :

1. Yamada, N., et al. Biochem. Biophys. Res. Commun. 192: 231-240. 2. Thomas, J.D., et al. 1993. Science. 261: 355-358. 3. Tamagnone, L., et al. Oncogene 9: 3683-3688.