BRAF | US Biological 030005 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

BRAF

catalog :

030005

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

13B83

reactivity :

human

product information

Catalog Number :

030005

Product wo Prefix :

BRAF

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

13B83

Isotype :

IgG1

Desc1 :

BRAF (V-raf murine sarcoma viral oncogene homolog B1) is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439). B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region, the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma, melanocytic nevi, primary cutaneous melanomas and colorectal cancers. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000 Western Blot: 1:500-1:2000 Immunohistochemistry: 1:200-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Alternate Name :

BRAF

Immunogen :

Purified recombinant fragment of human BRAF expressed in E. coli.

Specificity :

Recognizes human BRAF.

Purity :

Ascites

Form :

Supplied as a liquid, 0.03% sodium azide.

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 13B83 Host: mouse Source: human Concentration: Not Determined Form: Supplied as a liquid, 0.03% sodium azide. Purity: Ascites Immunogen: Purified recombinant fragment of human BRAF expressed in E. coli. Specificity: Recognizes human BRAF. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC WB

Storage Temperature :

-20°C

Reference :

1. Rapp, U.R., et al.1983. Proc. Natl. Acad .Sci. USA.80:4218-4222. 2. Kim J, Giuliano AE,Turner RR. 2006. Ann Surg. Nov, 244(5): 799-804. 3. Fullen DR, Poynter JN, Lowe L, 2006.Mod Pathol. 19(10): 1324-1332. 4. Terai K, Matsuda M. 2006. MBO J. 25(15):3556-3564. 5. Noda H, Kato Y, Yoshikawa H, 2006. J Exp Clin Cancer Res. 25(2):235-242.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/44/030005_1.jpg

Picture4 Text :

Immunohistochemical analysis of paraffin-embedded human testis tissues of BRAF using 030005