ATXN1 (Ataxin-1, ATX1) | US Biological 029977 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

ATXN1 (Ataxin-1, ATX1)

catalog :

029977

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

13B55

reactivity :

human

product information

Catalog Number :

029977

Product wo Prefix :

ATXN1 (Ataxin-1, ATX1)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

13B55

Isotype :

IgG1

Desc1 :

The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the `pure' cerebellar syndrome (SCA5), are most likely homogeneous disorders. Several SCA genes have been cloned and shown to contain CAG repeats in their coding regions. ADCA is caused by the expansion of the CAG repeats, producing an elongated polyglutamine tract in the corresponding protein. The expanded repeats are variable in size and unstable, usually increasing in size when transmitted to successive generations. The function of the ataxins is not known. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Flow Cytometry and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:2000 Immunohistochemistry (Paraffin): 1:200-1:1000 Immunofluorescence (IC): 1:200-1:1000 Flow Cytometry: 1:200-1:400 ELISA: 1:10,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Alternate Name :

ATX1; SCA1; D6S504E; ATXN1

Immunogen :

Purified recombinant fragment of human ATXN1 expressed in E. coli.

Specificity :

Recognizes human ATXN1

Purity :

Ascites

Form :

Ascites fluid, 0.03% sodium azide.

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 13B55 Host: mouse Source: human Concentration: Not Determined Form: Ascites fluid, 0.03% sodium azide. Purity: Ascites Immunogen: Purified recombinant fragment of human ATXN1 expressed in E. coli. Specificity: Recognizes human ATXN1 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IC IF IHC WB

Storage Temperature :

-20°C

Reference :

1. Nature. 2008 Apr 10;452(7188):713-8. 2. Biochem Biophys Res Commun. 2008 Jun 27;371(2):256-60. 3. Indian J Med Res. 2007 Nov;126(5):465-70.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/2/029977_1.png

Picture4 Text :

Immunofluorescence analysis of NTERA-2 cells using 029977 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin

Picture 5 File Name :

https://usbio-images.r.worldssl.net/prodimages/2/029977_5.png

Picture5 Text :

Flow cytometric analysis of Jurkat cells using 029977 (green) and negative control (purple)