HIstone H3.3 (H3F3A, H3.3A, H3F3, PP781, H3F3B, H3.3B) | US Biological 029502 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

HIstone H3.3 (H3F3A, H3.3A, H3F3, PP781, H3F3B, H3.3B)

catalog :

029502

quantity :

100 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

029502

Product wo Prefix :

HIstone H3.3 (H3F3A, H3.3A, H3F3, PP781, H3F3B, H3.3B)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone variant H3.3 is typically enriched in active chromatin. Applications: Suitable for use in Western Blot, Chromatin Immunoprecipitation, Dot Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml detected Histone H3.3 on 10ug of HeLa acid extract. Optimal dilutions to be determined by the researcher. Positive Control: HeLa acid extract Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mo

Immunogen :

Synthetic linear peptide corresponding to human Histone H3.3 (KLH).

Specificity :

Recognizes human Histone H3.3. Species Crossreactivity: mouse.

Purity :

Purified by affinity chromatography.

Form :

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

Concentration :

~1mg/ml

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: ~1mg/ml Form: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide. Purity: Purified by affinity chromatography. Immunogen: Synthetic linear peptide corresponding to human Histone H3.3 (KLH). Specificity: Recognizes human Histone H3.3. Species Crossreactivity: mouse. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

ChIP DB IC WB

Storage Temperature :

-20°C

Reference :

1. Kimura, H., et al. (2008). Cell Struct Funct. 33:61-73. 2. Nakagawa, T., et al. (2008). Genes Dev. 22:37-49. 3. Goldberg, A., et al. (2010). Cell. 140(5): 678-691.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/21/029502_1.jpg