Histone H3.3 (H3F3A, H3.3A, H3F3, PP781, H3F3B, H3.3B) (Biotin) | US Biological 029188 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Histone H3.3 (H3F3A, H3.3A, H3F3, PP781, H3F3B, H3.3B) (Biotin)

catalog :

029188

quantity :

100 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

biotin

reactivity :

human

product information

Catalog Number :

029188

Product wo Prefix :

Histone H3.3 (H3F3A, H3.3A, H3F3, PP781, H3F3B, H3.3B) (Biotin)

Host :

rabbit

Product Type :

Pab

Conjugate :

Biotin

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Recombinant corresponding to human Histone H3.3.

Specificity :

Recognizes human Histone H3.3.

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a liquid in 0.01M PBS, PH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with Biotin.

Concentration :

As reported

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in 0.01M PBS, PH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with Biotin. Purity: Purified by Protein G affinity chromatography. Immunogen: Recombinant corresponding to human Histone H3.3. Specificity: Recognizes human Histone H3.3. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

1. "Structure of a human histone cDNA: evidence that basally expressed histone genes have intervening sequences and encode polyadenylylated mRNAs." Wells D., Kedes L. Proc. Natl. Acad. Sci. U.S.A. 82:2834-2838 (1985). 2. "Unusual structure, evolutionary conservation of non-coding sequences and numerous pseudogenes characterize the human H3.3 histone multigene family." Wells D., Hoffman D., Kedes L. Nucleic Acids Res. 15:2871-2889 (1987). 3. "The human replacement histone H3.3B gene (H3F3B)." Albig W., Bramlage B., Gruber K., Klobeck H.-G., Kunz J., Doenecke D. Genomics 30:264-272 (1995).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/31/029188_1.jpg