antibody

14-3-3 eta (14-3-3 Protein eta, Protein AS1, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase Activation Protein eta Polypeptide, Tyrosine 3/tryptophan 5-monooxygenase Activation Protein eta Polypeptide, YWHA1, YWHAH, HGNC:12853)

0014-06A

100 ul

polyclonal

domestic rabbit

nonconjugated

14-3-3 eta (14-3-3 Protein eta, Protein AS1, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase Activation Protein eta Polypeptide, Tyrosine 3/tryptophan 5-monooxygenase Activation Protein eta Polypeptide, YWHA1, YWHAH, HGNC:12853)

www.usbio.net/antibodies/0014-06A/1433-eta-1433-Protein-eta-Protein-AS1-Tyrosine-3monooxygenasetryptophan-5monooxygenase-Activation-Protein-eta-Polypeptide-Tyrosine-3tryptophan-5monooxygenase-Activation-Protein-eta-Polypeptide-YWHA1-YWHAH-HGNC12853

rabbit

Pab

Antibodies

100 ul

IgG

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The initially described alpha and delta isoforms are confirmed to be phosphorylated forms of beta and zeta respectively. Through their amino-terminal alpha helical region, 14-3-3 proteins forms homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases and other signaling molecules. The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation independent interactions has been observed. 14-3-3 binding masks specific sequences of the target protein, and therefore, modulates target protein localization, phosphorylation state, stability and molecular interactions. 14-3-3 proteins may also induce target protein conformational changes which modify target protein function. Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in develop- ment and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform differ- ent functions despite their sequence similarities. Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes. Applications:. Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions:. Western Blot: 1:1000. Incubate membranes with diluted antibody, 1X TBS, 5% BSA, 0.1% Tween-20 at 4 C with gentle shaking overnight. Immunoprecipitation: 1:50 . Optimal dilutions to be determined by the researcher. Storage and Stability:. May be stored at 4 C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap

Hu Mk Mo Rt

Synthetic peptide corresponding to human 14-3-3 eta.

Recognizes endogenous levels of human total 14-3-3 eta protein at ~28kD. Show weak cross-reactivity with 14-3-3 gamma but does not detect any other 14-3-3 family isoforms. Species Crossreactivity: monkey, mouse and rat

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not Determined

Product Type: Pab. Isotype: IgG. Host: rabbit. Source: human. Concentration: Not Determined. Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to human 14-3-3 eta. Specificity: Recognizes endogenous levels of human total 14-3-3 eta protein at ~28kD. Show weak cross-reactivity with 14-3-3 gamma but does not detect any other 14-3-3 family isoforms. Species Crossreactivity: monkey, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IP WB

-20 C

1. Muslin, A.J. and Xing, H. (2000) Cell Signal 12, 703-9. 2. Mackintosh, C. (2004) Biochem. J. 381, 329-42. 3. Dougherty, M.K. and Morrison, D.K. (2004) J. Cell Sci. 117, 1875-84. 4. Yaffe, M.B. (2002) FEBS Lett. 513, 53-7. 5. Bridges, D. and Moorhead, G.B. (2004) Sci. STKE 2004, re10.

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