EIF4EBP1, phosphorylated (Thr36) (Eukaryotic Translation Initiation Factor 4E-binding Protein 1, 4E-BP1, eIF4E-binding Protein 1, Phosphorylated Heat- and Acid-stable Protein Regulated by Insulin 1, PHAS-I) discontinued | US Biological 0004-21K product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

EIF4EBP1, phosphorylated (Thr36) (Eukaryotic Translation Initiation Factor 4E-binding Protein 1, 4E-BP1, eIF4E-binding Protein 1, Phosphorylated Heat- and Acid-stable Protein Regulated by Insulin 1, PHAS-I) discontinued

catalog :

0004-21K

quantity :

50 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

0004-21K

Product wo Prefix :

Host :

rabbit

Product Type :

Pab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

50 ug

Isotype :

IgG

Desc1 :

4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12kD acidic protein that compete with eIF4G for binding of eiF4E to the mRNA 5 cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP. Non-phosphorylated 4E- BP1 will bind strongly to eiF4E while, the phosphorylated form will no (2)t. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 65. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot:1:500-1:1,000 Immunohistochemistry:1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mo Rt

Immunogen :

Synthetic phosphopeptide corresponding to human 4E-BP1 around the phosphorylation site of threonine 36 (S-T-TP-P-G).

Specificity :

Recognizes human 4E Binding Protein 1 when phosphorylated at (Thr36). Species Cross-Reactivity: mouse, rat.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

Concentration :

As reported

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to human 4E-BP1 around the phosphorylation site of threonine 36 (S-T-TP-P-G). Specificity: Recognizes human 4E Binding Protein 1 when phosphorylated at (Thr36). Species Cross-Reactivity: mouse, rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IHC WB

Storage Temperature :

-20°C