4E Binding Protein 1, phosphorylated (Thr70) (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP) | US Biological 0004-20A product information

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product summary

company name :

US Biological

product type :

antibody

product name :

4E Binding Protein 1, phosphorylated (Thr70) (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)

catalog :

0004-20A

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

0004-20A

Product wo Prefix :

4E Binding Protein 1, phosphorylated (Thr70) (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)

Host :

rabbit

Product Type :

Pab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

4E-BP1 (also known as PHAS-1) normally binds eIF4E, inhibiting cap-dependent translation. Hyperphosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. The PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1. 4E-BP1 is phosphorylated in vivo on multiple residues; phosphorylation by FRAP/mTOR on Thr37 and Thr46 of human 4E-BP1 may prime it for subsequent phosphorylation at sites including Ser65 and Thr70. Applications: Suitable for use in Western Blot, and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blotting: 1:1000 (Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.) Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Molecular Weight: 15-20kD Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mo Rt

Immunogen :

Synthetic phosphopeptide derived from the sequence of mouse 4E-BP1.

Specificity :

Detects endogenous levels of 4E-BP1 only when phosphorylated at Thr70. Does not detect 4E-BP1 phosphorylated at other sites. Species Cross-Reactivity: human, mouse, rat.

Purity :

Purified by Protein A affinity chromatography.

Form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: mouse Concentration: Not determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA 50% glycerol. No preservative added. Purity: Purified by Protein A affinity chromatography. Immunogen: Synthetic phosphopeptide derived from the sequence of mouse 4E-BP1. Specificity: Detects endogenous levels of 4E-BP1 only when phosphorylated at Thr70. Does not detect 4E-BP1 phosphorylated at other sites. Species Cross-Reactivity: human, mouse, rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IP WB

Storage Temperature :

-20°C

Reference :

(1) Pause, A. et al. (1994) Nature 371, 762–767. (2) Brunn, G.J. et al. (1997) Science 277, 99–101. (3) Gingras, A.C. et al. (1998) Genes Dev. 12, 502–513. (4) Fadden, P. et al. (1997) J. Biol. Chem. 272, 10240–10247. (5) Gingras, A.C. et al. (1999) Genes Dev. 13, 1422–1437.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/27/0004-20A_1.jpg