High sensitive mouse insulin immunoassay kit | Toronto BioScience 32270 product information

product summary

company name :

Toronto BioScience

product type :

ELISA/assay

product name :

High sensitive mouse insulin immunoassay kit

catalog :

32270

quantity :

0.2 - 7.0 ng/ml

price :

325 USD

more info or order :

Toronto BioScience product webpage

citations: 2

Reference
Lin Z, Tian H, Lam K, Lin S, Hoo R, Konishi M, et al. Adiponectin mediates the metabolic effects of FGF21 on glucose homeostasis and insulin sensitivity in mice. Cell Metab. 2013;17:779-89 pubmed publisher
Cheng K, Lam K, Wu D, Wang Y, Sweeney G, Hoo R, et al. APPL1 potentiates insulin secretion in pancreatic β cells by enhancing protein kinase Akt-dependent expression of SNARE proteins in mice. Proc Natl Acad Sci U S A. 2012;109:8919-24 pubmed publisher

product information

Catalog Number :

32270

Product Name :

High sensitive mouse insulin immunoassay kit

Host Species :

Mouse

Clonality :

Monoclonal

Conjugation :

Detection: conjugated to horse-radish peroxidase

Product Type :

ELISA kit

Size :

0.2 - 7.0 ng/ml

List Price (USD) :

325 USD

list of Pubmed id :

22566644, 23663741

Product Description :

This high sensitive mouse insulin ELISA kit is designed for the quantitative determination of insulin in mouse serum, plasma and fluid.

CrossReactivity :

Percent of cross reactivity: Human insulin = 100%, Rat insulin = 100%

SpeciesSummary :

Mouse specificity

ALTnames :

Ins-1

Specificity :

Mouse

Buffer :

10x Wash Buffer & a ready to use Assay Buffer are provided.

Uses :

ELISA: For the quantitative determination of insulin in mouse serum, plasma and fluid.

Application Summary :

This assay is a two-site ELISA. The micro-plate is pre-coated with a monoclonal antibody against insulin. Standards and samples are added into the wells and coincubated with a monoclonal antibody conjugated to horseradish peroxidase (HRP) enzyme. After wash step to remove any unbound substances, TMB substrate is added and colour develops in proportion to the amount of insulin bound initially. The assay is stopped and the optical density of the wells determined using a micro-plate reader. Since the increases in absorbance are directly proportional to the amount of captured insulin, the unknown sample concentration can be interpolated from a reference curve included in each assay.

Storage :

The kit should be stored at 2-8°C upon receipt. Remove any unused antibodycoated strips from the micro-plate, return them to the foil pouch and re-seal. Once opened, the strips may be stored at 2-8°C for up to one month.

Product References :

PNAS (USA) 2012 [109(23):8919-24]; Cell Metabolism 2013 [17(5):779-89]

more info or order :

Toronto BioScience product webpage