Toronto BioScience

ELISA/assay

Mouse FGF-21 Immunoassay Kit

32180

31.2- 2000 pg/ml

429 USD

32180

Mouse FGF-21 Immunoassay Kit

Rabbit

Polyclonal

Detection antibody: conjugated to biotin

ELISA kit

31.2- 2000 pg/ml

429 USD

26635356, 26294388, 26091256, 25055037

This mouse fibroblast growth factor 21 (FGF-21) ELISA kit is designed for quantification of mouse FGF-21 in serum, plasma and cell culture samples.

Human FGF-21: Yes, Mouse Mup-1: No, Mouse LCN2: No, Mouse Adiponectin: No, Mouse ANGPL4: No

Mouse specificity

Fibroblast growth factor 21(FGF-21) is a novel protein that has been implicated in the regulation of lipid and glucose metabolism under fasting and ketotic conditions1,2. In murine models, FGF-21 is predominantly expressed in liver, but it also expressed in adipose tissue and pancreatic β- cells3,4. FGF-21 stimulates glucose uptake in adipocytes. It also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents5. When administered daily for 6 weeks to diabetic rhesus monkeys, FGF-21 caused a dramatic decline in fasting plasma glucose, fructosamine, triglycerides, insulin, and glucagon6. Furthermore, elevated plasma FGF-21 concentrations in humans appear to be related to the presence of hepatic and peripheral insulin resistance7.

Fibroblast growth factor 21, FGF-21

Mouse

5x Assay Buffer & 10x Wash Buffer are provided.

This mouse FGF-21 ELISA kit is designed for quantification of mouse FGF-21 in serum, plasma and cell culture samples.

This assay is a quantitative sandwich ELISA. The immunoplate is pre- coated with a rabbit polyclonal antibody specific for mouse FGF-21. Standards and samples are pipetted into the wells and any mouse FGF-21 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for mouse FGF-21 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of mouse FGF-21 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured mouse FGF-21, the unknown sample concentration can be interpolated from a reference curve included in each assay.

The kit should be stored at 2-8°C upon receipt, and all reagents should be equilibrated to room temperature before use. Remove any unused antibody- coated strips from the mouse FGF-21 microplate, return them to the foil pouch and re-seal. Once opened, the strips may be stored at 2-8°C for up to one month.

[1] Kharitonenkov A, et al. (2005) J Clin Invest; 115: 1627– 1635. [2] Badman MK, Pissios P, et al. (2007) Cell Metab; 5: 426– 437. [3] Nishimura T, et al. (2000) Biochim Biophys Acta; 1492: 203– 206. [4] Kurosu H, et al. (2007) J Biol Chem; 282: 26687– 26695. [5] Kharitonenkov A, et al. (2005) J. Clin. Invest; 115: 1627–35. [6] Kharitonenkov, et al. (2007) Endocrinology; 148:774-81. [7] Chavez AO, et al. (2009) Diabetes Care; 32:1542-6.

Diabetes. 2014 Dec;63(12):4064-75., Diabetes 2012 [61(4):797-806].