Toronto BioScience

ELISA/assay

Mouse Major urinary protein-1 Immunoassay Kit

32150

0.625 - 40 ng/ml

455 USD

32150

Mouse Major urinary protein-1 Immunoassay Kit

Mouse

Polyclonal

Detection: conjugated to horse-radish peroxidase

ELISA kit

0.625 - 40 ng/ml

455 USD

This Mup1 ELISA kit is designed for quantification of mouse Mup1 in serum and plasma samples.

The antibodies used in this assay are specific to mouse Mup1.

Mouse specificity

Major urinary protein 1 (Mup1), also known as Mup7, Up-1, Ltn-1, Mup-1, Mup-a, Mup10 and Lvtn-1, is a low molecular weight secreted protein produced predominantly from the liver. [1] Structurally it belongs to the lipocalin family, which carries small hydrophobic ligands such as pheromones. It has been demonstrated that Mup1 is an important player in regulating energy expenditure and metabolism in mice. In both dietary and genetic obese mice, the circulating concentrations of Mup1 were markedly decreased compared with the lean controls. Replenishment of recombinant MUP-1 led to improved glucose tolerance and insulin sensitivity, as well as increased energy expenditure and locomotor activity in db/db diabetic mice. [2] It was suggested that Mup1 also regulates systemic glucose and/or lipid metabolism through the paracrine/autocrine regulation of the hepatic gluconeogenic and/or lipogenic programs, respectively. [3]

Major urinary protein 1 (Mup1), also known as Mup7, Up-1, Ltn-1, Mup-1, Mup-a, Mup10 and Lvtn-1

Mouse

5x Assay Buffer & 10x Wash Buffer are provided.

ELISA: This Mup1 ELISA kit is designed for quantification of mouse Mup1 in serum and plasma samples.

This assay is a sandwich ELISA designed for the quantitative detection of mouse Mup1 in samples. The immunoplate is pre-coated with antibody specific to mouse Mup1. Standards and samples are pipetted into the wells and any mouse Mup1 present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked polyclonal antibody specific for mouse Mup1 is added to the wells. After a final wash step, an HRP substrate solution is added and colour develops in proportion to the amount of mouse Mup1 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured mouse Mup1, the unknown sample concentration can be interpolated from a reference curve included in each assay.

The kit should be stored at 2-8°C upon receipt, and all reagents should be equilibrated to room temperature before use. Remove any unused antibody-coated strips from the Mup1 microplate, return them to the foil pouch and re-seal. Once opened, the strips may be stored at 2-8°C for up to one month.

1. McIntosh, I., and Bishop, J. O. (1989) Mol. Cell. Biol. 9, 2202–2207. 2. Hui X, Zhu W, Wang Y, et al. (2009) J Biol Chem. 284(21):14050-7. 3. Zhou Y, Jiang L, Rui L. (2009) J Biol Chem. 284(17):11152-9.