Wide Range Mouse Insulin Immunoassay Kit | Toronto BioScience 32100 product information

product summary

company name :

Toronto BioScience

product type :

ELISA/assay

product name :

Wide Range Mouse Insulin Immunoassay Kit

catalog :

32100

quantity :

1 - 50 ng/ml

price :

299 USD

more info or order :

Toronto BioScience product webpage

citations: 4

Reference
Durning S, Flanagan-Steet H, Prasad N, Wells L. O-Linked β-N-acetylglucosamine (O-GlcNAc) Acts as a Glucose Sensor to Epigenetically Regulate the Insulin Gene in Pancreatic Beta Cells. J Biol Chem. 2016;291:2107-18 pubmed publisher
Stiernet P, Guiot Y, Gilon P, Henquin J. Glucose acutely decreases pH of secretory granules in mouse pancreatic islets. Mechanisms and influence on insulin secretion. J Biol Chem. 2006;281:22142-51 pubmed
Jiang F, Mehta M, Morahan G. Quantification of insulin gene expression during development of pancreatic islet cells. Pancreas. 2010;39:201-8 pubmed publisher
Naito T, Kuma A, Mizushima N. Differential contribution of insulin and amino acids to the mTORC1-autophagy pathway in the liver and muscle. J Biol Chem. 2013;288:21074-81 pubmed publisher

product information

Catalog Number :

32100

Product Name :

Wide Range Mouse Insulin Immunoassay Kit

Host Species :

Mouse

Clonality :

Monoclonal

Conjugation :

Detection: conjugated to horse-radish peroxidase

Product Type :

ELISA kit

Size :

1 - 50 ng/ml

List Price (USD) :

299 USD

list of Pubmed id :

26598517, 23744068, 19812524, 16760469

Product Description :

This wide range mouse insulin ELISA kit is designed for the quantitative determination of insulin in mouse serum, plasma and fluid.

CrossReactivity :

Human insulin: 100%, Rat Insulin: 100%

SpeciesSummary :

Mouse specificity

ALTnames :

Ins-1

Specificity :

Mouse

Buffer :

10x Wash Buffer & a ready to use Assay Buffer are provided.

Uses :

ELISA: For the quantitative determination of insulin in mouse serum, plasma and fluid.

Application Summary :

This assay is a two-site ELISA. The micro-plate is pre-coated with a monoclonal antibody against insulin. Standards and samples are added into the wells and co- incubated with a monoclonal antibody conjugated to horseradish peroxidase (HRP) enzyme. After wash step to remove any unbound substances, TMB substrate is added and colour develops in proportion to the amount of insulin bound initially. The assay is stopped and the optical density of the wells determined using a micro-plate reader. Since the increases in absorbance are directly proportional to the amount of captured insulin, the unknown sample concentration can be interpolated from a reference curve included in each assay.

Storage :

The kit should be stored at 2-8°C upon receipt. Remove any unused antibody- coated strips from the micro-plate, return them to the foil pouch and re-seal. Once opened, the strips may be stored at 2-8°C for up to one month.

General References :

[1] Durning, SP. et al. (2016) J Biol Chem. 291(5):2107-18. [2] Naito, T. et al. (2013) J Biol Chem. 288(29):21074-81. [3] Cheng, KK. Et al. (2013) Biochem. 455(2):207-16. [4] Shungin, D. et al. (2015) Nature. 518:187–196.

Product References :

PNAS (USA) 2012 [109(23):8919-24]

more info or order :

Toronto BioScience product webpage