Toronto BioScience

ELISA/assay

Mouse Adiponectin Immunoassay Kit

32010

3.12-200 ng/ml

325 USD

32010

Mouse Adiponectin Immunoassay Kit

Mouse

Polyclonal

Standard: Purified, Detection: conjugated to horse-radish peroxidase

ELISA kit

3.12-200 ng/ml

325 USD

26417088, 26166748, 25915851, 25961840, 25071026

This Mouse Adiponectin ELISA kit is designed for quantification of mouse adiponectin in serum, plasma, adipocyte extracts and cell culture media.

No cross-reactivity with human adiponectin, and other cytokine or hormone molecules.

Mouse specificity

As described in the product data sheet, adiponectin, also known as apM1, Acrp30, GBP28 and adipoQ, is a circulating hormone predominantly produced from adipose tissue. Many pharmacological studies demonstrated that this protein possesses potent anti-diabetic, anti-atherogenic and anti-inflammatory functions. Supplement of adiponectin protein can decrease blood glucose, improve insulin sensitivity, alleviate fatty liver and prevent atherosclerosis. The protein is posttranslationally modified by hydroxylation and glycosylation, and forms three different oligomeric complexes in the circulation. Many clinical studies demonstrated that plasma adiponectin is an useful biomarker for metabolic syndrome, nonalcoholic steatohepatitis and certain type of cancers. Decreased circulating levels of plasma adiponectin (hypoadiponectinaemia) are associated with increased body mass index (BMI), decreased insulin sensitivity, less favourable plasma lipid profiles, increased levels of inflammatory markers and increased risk for the development of type 2 diabetes, hypertension, and coronary heart diseases. Low adiponectin concentrations were found to be predictive of a future reduction in insulin sensitivity and cardiovascular disorders. Administration of the anti-diabetic drugs thiazolidinediones (TZDs) raises circulating adiponectin levels. In addition, low plasma adiponectin levels are also associated with nonalcoholic steatohepatitis (NASH) and certain types of cancers.

Adiponectin is also known as apM1, Acrp30, GBP28 and adipoQ.

Mouse

5x Assay Buffer & 10x Wash Buffer are provided.

ELISA: This Mouse Adiponectin ELISA kit is designed for quantification of mouse adiponectin in serum, plasma, adipocyte extracts and cell culture media.

This assay is a sandwich ELISA using affinity-purified polyclonal antibodies against mouse adiponectin. The immunoplate is pre-coated with anti-mouse adiponectin capture antibody. Standards and samples are pipetted into the wells and any mouse adiponectin present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked polyclonal antibody specific for mouse adiponectin is added to the wells. After a final wash step, an HRP substrate solution is added and colour develops in proportion to the amount of mouse adiponectin bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured mouse adiponectin, the unknown sample concentration can be interpolated from a reference curve included in each assay.

The kit should be stored at 2-8°C upon receipt, and all reagents should be equilibrated to room temperature before use. Remove any unused antibody-coated strips from the mouse adiponectin microplate, return them to the foil pouch and re-seal. Once opened, the strips may be stored at 2- 8°C for up to one month.

[1] Trujillo ME, Scherer PE. (2005) J Intern Med. 257:167-175. [2] Berg AH. et al. (2001) Nat Med. 7:947-953. [3] Yamauchi T. et al.(2001) Nat Med. 7:941-946. [4] Xu A. et al. (2003) J Clin Invest, 112:91-100. [5] Okamoto Y. et al. (2002) Circulation. 106:2767-2770. [6] Wang Y. et al. (2002) J Biol Chem. 277:19521-19529. [7] Xu A. et al. (2005) J Biol Chem. 280:18073-18080. [8] Maeda N. et al. (2001) Diabetes. 50:2094-2099.

Cell Metabolism 2013 [17(5):779-89], Cell Metabolism 2015 Aug 4;22(2):279-90, Proc Natl Acad Sci U S A. 2014 Nov 4;111(44):15810-5