Toronto BioScience

ELISA/assay

Rapid Human Cystatin C Immunoassay Kit

31241

0.23 - 15 ng/ml

325 USD

31241

Rapid Human Cystatin C Immunoassay Kit

Mouse

Monoclonal

Detection: conjugated to horse-radish peroxidase

ELISA kit

0.23 - 15 ng/ml

325 USD

This Rapid Human Cystatin C ELISA kit is designed for quantification of human cystatin c in serum, plasma and urine samples.

No cross-reactivity with mouse and rat cystatin c, and other cytokine or hormone molecules.

Human specificity

Human cystatin c (or cystatin 3), which is composed of 120 amino acid residues, belongs to the cystatins superfamilly that inactivates lysosomal cysteine proteinases. As a strongly cationic and low-molecular weight (13.4 kDa) protein, it is almost freely filtered across the glomerular membrane, and is mainly used as a biomarker of kidney function. A growing body of evidence suggests that cystatin c is a more reliable biomarker of glomerular filtration rate than creatinine [1-3]. In addition to kidney disease, altered serum levels of cystatin c are associated with several types of cardiovascular disease, including myocardial infarction, stroke, heart failure, peripheral arterial disease and metabolic syndrome [4-7] . It also seems to play a role in brain disorders involving amyloid, such as Alzheimer's disease [8, 9]. Furthermore, Cystatin c has also been investigated as a prognostic marker in several forms of cancer [11, 12].

Cystatin C

Human

5x Assay Buffer & 10x Wash Buffer are provided.

ELISA: This Rapid Human Cystatin C ELISA kit is designed for quantification of human cystatin c in serum, plasma and urine samples.

This assay is a sandwich ELISA designed for the quantitative detection of human cystatin c in samples in 1 hour. The immunoplate is pre-coated with antibody specific to human cystatin c. Standards and samples are pipetted into the wells and any human cystatin c present is sandwiched by the immobilised antibody and a second horseradish peroxidase (HRP)-linked antibody specific to human cystatin c that is co-incubated with the samples. After wash step to remove any unbound substances, the HRP substrate solution is added and colour develops in proportion to the amount of human cystatin c bound initially. The assay is stopped and the optical density of the wells determined using a micro-plate reader. Since the increases in absorbance are directly proportional to the amount of captured human cystatin c, the unknown sample concentration can be interpolated from a reference curve included in each assay.

The kit should be stored at 2-8°C upon receipt, and all reagents should be equilibrated to room temperature before use. Remove any unused antibodycoated strips from the Human cystatin c micro-plate, return them to the foil pouch and re-seal. Once opened, the strips may be stored at 2-8°C for up to one month.

[1] Stevens LA, Coresh J, Schmid CH, et al. (2008) Am J Kidney Dis. 51:395–406. [2] Dharnidharka VR, Kwon C, Stevens G. (2002) Am. J. Kidney Dis. 40 (2): 221–6. [3] Hermida J, Tutor JC. (2006) Ther Drug Monit. 28 (3): 326–31. [4] Zethelius B, Berglund L, Sundström J, et al. (2008) N. Engl. J. Med. 358 (20): 2107–16. [5] Ix JH, Shlipak MG, Chertow GM, Whooley MA. (2007) Circulation 115 (2): 173–9. [6] Deo R, Fyr CL, Fried LF, et al. (January 2008) Am. Heart J. 155 (1): 62–8.[7] Servais A, Giral P, Bernard M, et al. (2008) Am. J. Med. 121 (5): 426–32. [8]Mi W, Pawlik M, Sastre M, et al. (2007) Nat. Genet. 39 (12): 1440–2. [9] Kaeser SA, Herzig MC, Coomaraswamy J, et al. (2007) Nat. Genet. 39 (12): 1437–9. [10] Zurdel J, Finckh U, Menzer G, et al. (2002) Br J Ophthalmol 86 (2): 214–9. [11] Strojan P, Oblak I, Svetic B, et al. (2004) Br. J. Cancer 90 (10): 1961–8. [12] Kos J, Krasovec M, Cimerman N, et al. (2000) Clin. Cancer Res. 6 (2): 505–11.