Toronto BioScience

ELISA/assay

High sensitive human C-Reactive Protein (CRP) Immunoassay Kit

31220

0.312-20 ng/ml

299 USD

31220

High sensitive human C-Reactive Protein (CRP) Immunoassay Kit

Mouse

Monoclonal

Detection: conjugated to horse-radish peroxidase

ELISA kit

0.312-20 ng/ml

299 USD

23525430, 25189569

This Human CRP ELISA kit is designed for quantification of human CRP in serum and plasma.

No cross-reactivity with mouse and rat CRP, and other cytokine or hormone molecules.

Human specificity

C-reactive protein (CRP) is a circulating protein mainly secreted from the liver. This acute phase protein consists of five identical non-glycosylated subunits of 23 kDa, that give rise to a symmetrically arranged globular protein with molecular weight of approximately 120 kDa. [1] It has long been recognized that CRP is closely related to immunology, inflammation and host defence; as a result it has been used as an inflammatory marker. However, the development of high-sensitivity CRP (hsCRP) ELISA had addressed its role in other clinical issues. There is accumulating evidence suggesting the important role that CRP plays in mediating cardiovascular diseases (CVD) and type 2 diabetes.[2-4] Normally CRP is presenting only in a trace amount in circulation ( 3 g/ml is considered to have low, moderate and high risk, respectively, of CVD and myocardial infraction. [7] Therefore, blood CRP level has become a promising measure of CVD risk. [8,9]

C-reactive protein (CRP)

Human

5x Assay Buffer & 10x Wash Buffer are provided.

ELISA: This Human CRP ELISA kit is designed for quantification of human CRP in serum and plasma.

This assay is a quantitative sandwich ELISA using monoclonal antibodies against human CRP. The immunoplate is pre-coated with a monoclonal antibody specific for human CRP. Standards and samples are pipetted into the wells and any human CRP present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked monoclonal antibody specific for human CRP is added to the wells. After wash step to remove any unbound reagents, an HRP substrate solution is added and colour develops in proportion to the amount of human CRP bound initially. The assay is stopped and the optical density of the wells determined using a micro-plate reader. Since the increases in absorbance are directly proportional to the amount of captured human CRP, the unknown sample concentration can be interpolated from a reference curve included in each assay.

The kit should be stored at 2-8°C upon receipt, and all reagents should be equilibrated to room temperature before use. Remove any unused antibodycoated strips from the micro-plate, return them to the foil pouch and re-seal. Once opened, the strips may be stored at 2-8°C for up to one month.

1. Thompson D., Pepys M.B. and Wood S.P. (1999) Structure, 7, 169- 177. 2. Festa A, D’Agostino R. Jr., Tracy R.P. and Haner S.M. (2002) Diabetes, 51, 1131-1137. 3. Verma S. and Yeh E.T. (2003) Am J Physiol, 285, R1253-R1258. 4. Jialal I., Devaraj S. and Venugopal S.K. (2004) Hypertension, 44, 6- 11. 5. Kindmark C.O. (1972) Scand J Clin Lab Invest, 29, 407-411. 6. Macy E.M., Hayes T.E. and Tracy R.P. (1997) Clin Chem, 43, 52-58. 7. Ridker P.M. (2004) Am Heart Hosp J, 2 (4 Suppl 1), 4-9. 8. Benzaquen l.R., Yu H. and Rifai N. (2002) Crit Rev Clin Lab Sci, 39, 459-497. 9. Pearson T.A. et al., (2003) Circulation, 107, 499-511.

Journal American Heart Association 2013 [2(1):e004176]; Arterioscler Thromb Vasc Biol. 2014 Nov;34(11):2457-64