Toronto BioScience

ELISA/assay

Human FGF-19 Immunoassay Kit

31200

31.2-2000 pg/ml

390 USD

31200

Human FGF-19 Immunoassay Kit

Mouse

Monoclonal

Detection: Biotin-labeled

ELISA kit

31.2-2000 pg/ml

390 USD

23628619

This human FGF-19 ELISA kit is designed for quantification of human FGF- 19 in serum, plasma and cell culture supernate samples.

No cross-reactivity with human Adiponectin, FGF-21, FABP4, LCN2, RBP4 and PAI-1.

Human specificity

Fibroblast growth factor 19 (FGF-19) is a member of a subfamily of FGFs that includes FGF-21 and FGF-23, each member functions as an important regular of nutrient metabolism [1]. The primary source of endocrine FGF-19 is the ileum, bile acids release into the intestine after a meal to induce expression of FGF-19 [2]. Circulating FGF-19 plays an important role in maintaining proper bile acid homeostasis [3]. Several pharmacologic studies in hyperglycaemic, obese animal models have shown that FGF-19 can improve metabolic rate and lower serum glucose and hepatic triglyceride and cholesterol levels [4][5]. Like insulin, FGF-19 functions as postprandial hormone to govern hepatic protein synthesis, glycogen synthesis and gluconeogenesis, but does not stimulate lipogenesis [6].

Fibroblast growth factor 19 (FGF-19)

Human

5x Assay Buffer & 10x Wash Buffer are provided.

ELISA: This human FGF-19 ELISA kit is designed for quantification of human FGF- 19 in serum, plasma and cell culture supernate samples.

This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a rabbit polyclonal antibody specific for human FGF-19. Standards and samples are pipetted into the wells and any human FGF-19 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human FGF-19 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human FGF-19 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human FGF-19, the unknown sample concentration can be interpolated from a reference curve included in each assay

The kit should be stored at 2-8°C upon receipt, and all reagents should be equilibrated to room temperature before use. Remove any unused antibodycoated strips from the human FGF-19 microplate, return them to the foil pouch and re-seal. Once opened, the strips may be stored at 2-8°C for up to one month.

[1] Beenken A, et al. (2009) Nat Rev Drug Discov; 8: 235– 253. [2] Inagaki T, et al. (2005) Cell Metab; 2: 217– 225. [3] Lundasen T, et al. (2006) J Inter Med;260:530-536. [4] Tomlinston E, et al. (2002) Endocrinology; 143: 1741-1747. [5] Fu L, et al. (2004) Endocrinology; 145: 2594-2603. [6] Kir S, et al. (2011) Science; 331: 1621– 1624.

Arterioscler Thromb Vasc Biol. 2014 Nov;34(11):2457-64Diabetes Care 2013 [36(9):2810-4];