Human FGF-21 Immunoassay Kit | Toronto BioScience 31180 product information

product summary

company name :

Toronto BioScience

product type :

ELISA/assay

product name :

Human FGF-21 Immunoassay Kit

catalog :

31180

quantity :

30-1920 pg/ml

price :

390 USD

more info or order :

Toronto BioScience product webpage

citations: 3

Reference
Singhal G, Fisher F, Chee M, Tan T, El Ouaamari A, Adams A, et al. Fibroblast Growth Factor 21 (FGF21) Protects against High Fat Diet Induced Inflammation and Islet Hyperplasia in Pancreas. PLoS ONE. 2016;11:e0148252 pubmed publisher
Youm Y, Horvath T, Mangelsdorf D, Kliewer S, Dixit V. Prolongevity hormone FGF21 protects against immune senescence by delaying age-related thymic involution. Proc Natl Acad Sci U S A. 2016;113:1026-31 pubmed publisher
Badman M, Pissios P, Kennedy A, Koukos G, Flier J, Maratos-Flier E. Hepatic fibroblast growth factor 21 is regulated by PPARalpha and is a key mediator of hepatic lipid metabolism in ketotic states. Cell Metab. 2007;5:426-37 pubmed

product information

Catalog Number :

31180

Product Name :

Human FGF-21 Immunoassay Kit

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Detection antibody: conjugated to biotin

Product Type :

ELISA kit

Size :

30-1920 pg/ml

List Price (USD) :

390 USD

list of Pubmed id :

26872145, 26755598, 17550778

Product Description :

This human fibroblast growth factor 21 (FGF-21) ELISA kit is designed for the quantitative determination of human FGF-2 1 concentrations in serum, plasma and cell culture supernate samples.

CrossReactivity :

Mouse FGF-21: No, Human FABP4: No, Human LCN2: No, Human Adiponectin: No, Human ANGPL4: No

SpeciesSummary :

Human specificity

Background :

Fibroblast growth factor 21 (FGF-21) is a novel protein that has been implicated in the regulation of lipid and glucose metabolism under fasting and ketotic conditions[1,2]. In murine models, FGF-21 is predominantly expressed in liver, but it also expressed in adipose tissue and pancreatic β-cells[3,4]. FGF- 21 stimulates glucose uptake in adipocytes. It also protects animals from diet- induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents[5]. When administered daily for 6 weeks to diabetic rhesus monkeys, FGF-21 caused a dramatic decline in fasting plasma glucose, fructosamine, triglycerides, insulin, and glucagon[6]. Furthermore, elevated plasma FGF-21 concentrations in humans appear to be related to the presence of hepatic and peripheral insulin resistance[7].

ALTnames :

Fibroblast growth factor 21, FGF-21

Specificity :

Human

Buffer :

5x Assay Buffer & 10x Wash Buffer are provided.

Uses :

This human FGF-21 ELISA kit is designed for quantification of human FGF- 21 in serum, plasma and cell culture supernate samples.

Application Summary :

This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a rabbit polyclonal antibody specific for human FGF-21. Standards and samples are pipetted into the wells and any human FGF-21 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human FGF-21 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human FGF-21 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human FGF-21, the unknown sample concentration can be interpolated from a reference curve included in each assay.

Storage :

The kit should be stored at 2-8°C upon receipt, and all reagents should be equilibrated to room temperature before use. Remove any unused antibody- coated strips from the human FGF-21 microplate, return them to the foil pouch and re-seal. Once opened, the strips may be stored at 2-8°C for up to one month.

General References :

[1] Kharitonenkov A, et al. (2005) J Clin Invest; 115: 1627– 1635. [2] Badman MK, Pissios P, et al. (2007) Cell Metab; 5: 426– 437. [3] Nishimura T, et al. (2000) Biochim Biophys Acta; 1492: 203– 206. [4] Kurosu H, et al. (2007) J Biol Chem; 282: 26687– 26695. [5] Kharitonenkov A, et al. (2005) J. Clin. Invest; 115: 1627–35. [6] Kharitonenkov, et al. (2007) Endocrinology; 148:774-81. [7] Chavez AO, et al. (2009) Diabetes Care; 32:1542-6.

Product References :

Cell Metabolism 2013 [17(5):779-89]

more info or order :

Toronto BioScience product webpage