Toronto BioScience

ELISA/assay

Human RBP4 Immunoassay Kit

31060

3.12-200 ng/ml

325 USD

31060

Human RBP4 Immunoassay Kit

Mouse

Monoclonal

Detection: conjugated to horse-radish peroxidase

ELISA kit

3.12-200 ng/ml

325 USD

23144467, 23799122, 24800810

This Human RBP4 ELISA kit is designed for quantification of Human RBP4 in serum, plasma, and adipocyte extracts or cell culture media samples.

No cross-reactivity with mouse and rat RBP4, and other cytokine or hormone molecules.

Human specificity

Retinol binding protein 4(RBP4), originally known as a specific transport of retinol in blood, is also a novel inflammatory and insulin resistance marker [1]. Serum RBP4 levels are elevated in insulin-resistant mice and humans with obesity and type 2 diabetes [2,3]. Animal experiments found that increased secretion of RBP4 might reduces insulin-dependent glucose uptake by muscle tissue by reducing the activity of PI(3)K (phosphoinositide 3-kinase), and increased hepatic glucose output by increasing the expression of the enzyme PEPCK2 . Studies suggested that elevated serum RBP4 was associated with components of the metabolic syndrome, including increased body-mass index, waist-to-hip ratio, serum triglyceride levels, and systolic blood pressure and decreased highdensity lipoprotein cholesterol levels [4]. Furthermore, circulating RBP4 concentrations were associated with subclinical cardiovascular disease, which imply that RBP4 could be involved in the development of atherosclerosis [5].

RBP4 retinol binding protein 4

Human

5x Assay Buffer & 10x Wash Buffer are provided.

ELISA: This Human RBP4 ELISA kit is designed for quantification of Human RBP4 in serum, plasma, and adipocyte extracts or cell culture media samples.

This assay is a quantitative sandwich ELISA using monoclonal antibodies against human RBP4. The immunoplate is pre-coated with a monoclonal antibody specific for human RBP4 and the nonspecific binding sites are blocked. Standards and samples are pipetted into the wells and any Human RBP4 present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked monoclonal antibody specific for human RBP4 is added to the wells. After wash step to remove any unbound reagents, an HRP substrate solution is added and colour develops in proportion to the amount of human RBP4 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human RBP4, the unknown sample concentration can be interpolated from a reference curve included in each assay.

The kit should be stored at 2-8°C upon receipt, and all reagents should be equilibrated to room temperature before use. Remove any unused antibodycoated strips from the Human RBP4 microplate, return them to the foil pouch and re-seal. Once opened, the strips may be stored for up to one month at 2- 8°C.

[1] Shepherd PR, et al. (1999). N Engl J Med 341:248-257 [2] Yang Q, et al. (2005). Nature. 436:356–362. [3]. Cho YM, et al. (2006). Diabetes Care 29: 2457–2461. [4] Graham TE, et al. (2006). N Engl J Med 354: 2552–2563 [5] Ingelsson, E, et al. (2009). Diabetes Care 32: 733-735

Journal of Clinical Endocrinology & Metabolism 2012 [97(12):4701-8]; PLoS One 2013 [8(6):e66607]; PLoS One. 2014 May 6;9(5):e96489;