Toronto BioScience

ELISA/assay

Human Fatty-acid binding protein 4 Immunoassay Kit

31030

0.39- 25 ng/ml

520 USD

31030

Human Fatty-acid binding protein 4 Immunoassay Kit

Mouse

Monoclonal

Detection: Biotin-labeled

ELISA kit

0.39- 25 ng/ml

520 USD

23144467, 23013043, 23525430

This Human FABP4 ELISA kit is designed for quantification of Human FABP4 in serum, plasma, and adipocyte extracts or cell culture media samples.

No cross-reactivity with mouse and rat FABP4, and other cytokine or hormone molecules.

Human specificity

Fatty-acid binding protein 4 (FABP4), also termed adipocyte fatty-acid binding protein (A-FABP), or aP2, is a novel adipocyte-expressed factor which accounted for ~6% of total cellular proteins. Several animal experiments suggested that FABP-4 plays a key role in the link between obesity and various features of metabolic syndrome [1]. Mice with targeted disruption of FABP-4 accompany FABP-5 almost completely protect against diet-induced obesity, insulin resistance, dyslipidemia, type 2 diabetes, and fatty liver disease [2]. Studies in human found FABP-4 serum levels were significantly increased in overweight and obese subjects, which predicted the risk to develop a metabolic syndrome and type 2 diabetes [3,4]. Additionally, serum FABP-4 levels were associated with nonalcoholic fatty liver disease, carotid atherosclerosis and coronary artery disease [5-7] .

Fatty-acid binding protein 4 (FABP4), also termed adipocyte fatty-acid binding protein (A-FABP), or aP2, is a novel adipocyte-expressed factor which accounted for ~6% of total cellular proteins.

Human

5x Assay Buffer & 10x Wash Buffer are provided.

ELISA: This Human FABP4 ELISA kit is designed for quantification of Human FABP4 in serum, plasma, and adipocyte extracts or cell culture media samples.

This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a mouse monoclonal antibody specific for human FABP4. Standards and samples are pipetted into the wells and any Human FABP4 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human FABP4 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate is added, after the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human FABP4 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human FABP4, the unknown sample concentration can be interpolated from a reference curve included in each assay.

The kit should be stored at 2-8°C upon receipt, and all reagents should be equilibrated to room temperature before use. Remove any unused antibodycoated strips from the Human FABP4 microplate, return them to the foil pouch and re-seal. Once opened, the strips may be stored for up to one month at 2-8°C.

[1] Makowski L, et al. (2004) J Nutr. 134: 2464S–2468S. [2] Makowski L, et al. (2001) Nat Med. 7: 699–705. [3] Xu A, et al. (2006). Clin Chem. 52(3):405-13. [4] Xu A, et al. (2007). Circulation. 115:1537–1543. [5] Rhee EJ, et al. (2009) Eur J Endocrinol. 160(2):165-72. [6] Tso AW, et al. (2007) Diabetes Care. 30(10):2667-72 [7] J. Hyun Koh, et al. (2009) Diabetes Care. 32(1): 147 - 152.

Journal of Clinical Endocrinology & Metabolism 2012 [97(12):4701-8]; Clinical Science 2013 [124(2):115-22]; Journal American Heart Association 2013 [2(1):e004176];