Toronto BioScience

ELISA/assay

Rapid Human Adiponectin Immunoassay kit

31012

7.8-500 ng/ml

364 USD

31012

Rapid Human Adiponectin Immunoassay kit

Mouse

Monoclonal

Detection: conjugated to horse-radish peroxidase

ELISA kit

7.8-500 ng/ml

364 USD

23525430, 25189569

This Rapid Human Adiponectin ELISA kit is designed for quantification of human adiponectin in serum, plasma, and adipocyte extracts or cell culture media samples.

No cross-reactivity with mouse and rat adiponectin, and other cytokine or hormone molecules tested, including human resistin, TNF , ANGPTL4, insulin, leptin and IL6

Human specificity

Adiponectin, also known as apM1, Acrp30, GBP28 and adipoQ, is a circulating hormone predominantly produced from adipose tissue [1]. Many pharmacological studies demonstrated that this protein possesses potent anti-diabetic, antiatherogenic and anti-inflammatory functions. Supplement of adiponectin protein can decrease blood glucose [2], improve insulin sensitivity [3], alleviate fatty liver [4] and prevent atherosclerosis [5]. The protein is posttranslationally modified by hydroxylation and glycosylation [6], and forms three different oligomeric complexes in the circulation [7]. Many clinical studies demonstrated that plasma adiponectin is an useful biomarker for metabolic syndrome, nonalcoholic steatohepatitis and certain type of cancers 1 . Decreased circulating levels of plasma adiponectin ('hypoadiponectinaemia') are associated with increased body mass index (BMI), decreased insulin sensitivity, less favourable plasma lipid profiles, increased levels of inflammatory markers and increased risk for the development of type 2 diabetes, hypertension, and coronary heart diseases. Low adiponectin concentrations were found to be predictive of a future reduction in insulin sensitivity and cardiovascular disorders. Administration of the anti-diabetic drugs thiazolidinediones (TZDs) raises circulating adiponectin levels [8]. In addition, low plasma adiponectin levels are also associated with nonalcoholic steatohepatitis (NASH) and certain types of cancers

Adiponectin, also known as apM1, Acrp30, GBP28 and adipoQ

Human

5x Assay Buffer & 10x Wash Buffer are provided.

ELISA: This assay is a sandwich ELISA designed for the quantitative detection of human adiponectin in samples in 1 hour.

This assay is a sandwich ELISA designed for the quantitative detection of human adiponectin in samples in 1 hour. A mouse monoclonal antibody specific to human adiponectin has been pre-coated onto a micro-titre plate. The user pipettes standards and samples into the wells and any human adiponectin present is sandwiched by the immobilised antibody and a second horseradish peroxidase (HRP)-linked monoclonal antibody specific to human adiponectin that is co-incubated with the samples. After wash step to remove any unbound reagents, an HRP substrate solution is added and colour develops in proportion to the amount of human adiponectin bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human adiponectin, the unknown sample concentration can be interpolated from a reference curve included in each assay.

The kit should be stored at 2-8°C upon receipt, and all reagents should be equilibrated to room temperature before use. Remove any unused antibody-coated strips from the human adiponectin microplate, return them to the foil pouch and reseal. Once opened, the strips may be stored at 2-8°C for up to one month.

1. Trujillo ME, Scherer PE. (2005) J Intern Med. 257:167-175. 2. Berg AH. et al. (2001) Nat Med. 7:947-953. 3. Yamauchi T. et al.(2001) Nat Med. 7:941-946. 4. Xu A. et al. (2003) J Clin Invest, 112:91-100. 5. Okamoto Y. et al. (2002) Circulation. 106:2767-2770. 6. Wang Y. et al. (2002) J Biol Chem. 277:19521-19529. 7. Xu A. et al. (2005) J Biol Chem. 280:18073-18080. 8. Maeda N. et al. (2001) Diabetes. 50:2094-2099.

Journal American Heart Association 2013 [2(1):e004176]; Arterioscler Thromb Vasc Biol. 2014 Nov;34(11):2457-64