Toronto BioScience

ELISA/assay

Human Adiponectin Immunoassay kit

31010

1.56-100 ng/ml

325 USD

31010

Human Adiponectin Immunoassay kit

Mouse

Monoclonal

Detection: conjugated to horse-radish peroxidase

ELISA kit

1.56-100 ng/ml

325 USD

23525430, 25189569

This Human Adiponectin ELISA kit is designed for quantification of human adiponectin in serum, plasma, adipocyte extract and cell culture media samples.

No cross-reactivity with mouse and rat adiponectin, and other cytokine or hormone molecules including human resistin, TNF , ANGPTL4, insulin, leptin and IL6.

Human specificity

Adiponectin, also known as apM1, Acrp30, GBP28 and adipoQ, is a circulating hormone predominantly produced from adipose tissue [1] . Many pharmacological studies demonstrated that this protein possesses potent anti-diabetic, antiatherogenic and anti-inflammatory functions. Supplement of adiponectin protein can decrease blood glucose [2], improve insulin sensitivity [3], alleviate fatty liver [4] and prevent atherosclerosis [5]. The protein is post-translationally modified by hydroxylation and glycosylation[6], and forms three different oligomeric complexes in the circulation [7]. Many clinical studies demonstrated that plasma adiponectin is an useful biomarker for metabolic syndrome, nonalcoholic steatohepatitis and certain type of cancers [1]. Decreased circulating levels of plasma adiponectin (hypoadiponectinaemia) are associated with increased body mass index (BMI), decreased insulin sensitivity, less favourable plasma lipid profiles, increased levels of inflammatory markers and increased risk for the development of type 2 diabetes, hypertension, and coronary heart diseases. Low adiponectin concentrations were found to be predictive of a future reduction in insulin sensitivity and cardiovascular disorders. Administration of the anti-diabetic drugs thiazolidinediones (TZDs) raises circulating adiponectin levels8 . In addition, low plasma adiponectin levels are also associated with nonalcoholic steatohepatitis (NASH) and certain types of cancers.

Adiponectin, also known as apM1, Acrp30, GBP28 and adipoQ

Human

5x Assay Buffer & 10x Wash Buffer are provided.

ELISA: This Human Adiponectin ELISA kit is designed for quantification of human adiponectin in serum, plasma, adipocyte extract and cell culture media samples.

This assay is a quantitative sandwich ELISA using monoclonal antibodies against human adiponectin. The immunoplate is pre-coated with a monoclonal antibody specific for human adiponectin. Standards and samples are pipetted into the wells and any human adiponectin present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked monoclonal antibody specific for human adiponectin is added to the wells. After a final wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human adiponectin bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human adiponectin, the unknown sample concentration can be interpolated from a reference curve included in each assay.

The kit should be stored at 2-8°C upon receipt, and all reagents should be equilibrated to room temperature before use. Remove any unused antibodycoated strips from the Human adiponectin microplate, return them to the foil pouch and re-seal. Once opened, strips may be stored at 2-8°C for up to one month.

[1] Trujillo ME, Scherer PE. (2005) J Intern Med. 257:167-175. [2] Berg AH. et al. (2001) Nat Med. 7:947-953. [3] Yamauchi T. et al.(2001) Nat Med. 7:941-946. [4] Xu A. et al. (2003) J Clin Invest, 112:91-100. [5] Okamoto Y. et al. (2002) Circulation. 106:2767-2770. [6] Wang Y. et al. (2002) J Biol Chem. 277:19521-19529. [7] Xu A. et al. (2005) J Biol Chem. 280:18073-18080. [8] Maeda N. et al. (2001) Diabetes. 50:2094-2099.

Journal American Heart Association 2013 [2(1):e004176]; Arterioscler Thromb Vasc Biol. 2014 Nov;34(11):2457-64