Appl2 (human), rabbit polyclonal | Toronto BioScience 11140 product information

product summary

company name :

Toronto BioScience

product type :

antibody

product name :

Appl2 (human), rabbit polyclonal

catalog :

11140

quantity :

100 μg

price :

259 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA

more info or order :

Toronto BioScience product webpage

citations: 4

Reference
CHIAL H, Lenart P, Chen Y. APPL proteins FRET at the BAR: direct observation of APPL1 and APPL2 BAR domain-mediated interactions on cell membranes using FRET microscopy. PLoS ONE. 2010;5:e12471 pubmed publisher
Pyrzynska B, Banach-Orlowska M, Teperek-Tkacz M, Miekus K, Drabik G, Majka M, et al. Multifunctional protein APPL2 contributes to survival of human glioma cells. Mol Oncol. 2013;7:67-84 pubmed publisher
Rashid S, Pilecka I, Torun A, Olchowik M, Bielinska B, Miaczynska M. Endosomal adaptor proteins APPL1 and APPL2 are novel activators of beta-catenin/TCF-mediated transcription. J Biol Chem. 2009;284:18115-28 pubmed publisher
Barbieri M, Esposito A, Angellotti E, Rizzo M, Marfella R, Paolisso G. Association of genetic variation in adaptor protein APPL1/APPL2 loci with non-alcoholic fatty liver disease. PLoS ONE. 2013;8:e71391 pubmed publisher

product information

Catalog Number :

11140

Product Name :

Appl2 (human), rabbit polyclonal

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Purified

Product Type :

Antibody

Size :

100 μg

List Price (USD) :

259 USD

list of Pubmed id :

19433865, 22989406, 23977033, 20814572

Isotype :

Rabbit IgG

Product Description :

This rabbit polyclonal antibody can detect several types of APPL2 in different species such as human, monkey, mouse, rat etc. (about 75kDa)

CrossReactivity :

Monkey, Mouse, Rat

SpeciesSummary :

Human specificity, however, it can also detect monkey, mouse, and rat APPL1

Background :

APPL2, also termed as Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2, has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, APPL1, adiponectin receptors, and proteins of the NuRD/MeCP1 complex.

ALTnames :

Adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2) is also known as DIP13B

Immunogen :

Recombinant full-length human APPL2 expressed in E. coli cells

Specificity :

Human, Monkey, Mouse, Rat

Buffer :

Solution in PBS

Uses :

WB, Immunostaining, ELISA

Application Summary :

Western blot - This antibody can be used at 0.2 - 0.3 μg/mL with the appropriate secondary reagents to detect APPL2. Immunostaining - This antibody can be used at 2-3 μg/mL with the appropriate secondary reagents to detect APPL2. ELISA - This antibody can be used at 1.0 - 2.0 μg/mL with the appropriate secondary reagents to detect APPL2.

Storage :

Store at –20°C. For long-term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.

General References :

[1] Rashid S, et al. (2009) J Biol Chem; 284(27):18115-28. [2] Pyrzynska B, et al. (2013) Mol Oncol; 7(1)67-84. [3] Barbieri M, et al. (2013) PLoS One; 8(8):e71391.

Product References :

Int J Cardiol 2016 Aug [216: 32-42], Diabetes 2014 Nov; [63(11): 3748-3758]

more info or order :

Toronto BioScience product webpage