Toronto BioScience

antibody

Appl1 (human), rabbit polyclonal

11130

100 μg

259 USD

polyclonal

rabbit

nonconjugated

human, mouse

11130

Appl1 (human), rabbit polyclonal

Rabbit

Polyclonal

Purified

Antibody

100 μg

259 USD

22379109, 26459602, 24813896, 23977033, 22685300

Rabbit IgG

This rabbit polyclonal antibody can detect several types of APPL1 in different species such as human, monkey, mouse, rat etc. (about 85kDa)

Monkey, Mouse, Rat

Human specificity, however, it can also detect monkey, mouse, and rat APPL1

APPL1, an adaptor protein containing an NH2-terminal Bin/Amphiphiphysin/Rvs (BAR) domain, a central pleckstrin homology (PH) domain and a COOH-terminal phosphotyrosine binding (PTB) domain [1], was originally identified as an interacting partner of Akt in a yeast two-hybrid assay using Akt2 as a bait [2]. APPL1 binds to a number of cell surface receptors (TrkA[3, 4], DCC[5], adiponectin [6, 7], FSH[8]) and intracellular signaling molecules (small GTPase Rab5[9], GIPC[4] and inositol 5- phosphatase[10], suggesting that APPL1 may act as a common relay to coordinate diverse signaling cascades. APPL1 potentiates insulin-mediated Akt activation by counteracting the effect of the Akt inhibitor TRB3 [11].

Adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1) is also known as APPL; MODY14; DIP13alpha

Recombinant full-length human APPL1 expressed in E. coli

Human, Monkey, Mouse, Rat

Solution in PBS

WB, Immunostaining, ELISA, Immunoprecipitation

Western blot - This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect APPL1. Immunostaining - This antibody can be used at 1.0 -2.0 μg/mL with the appropriate secondary reagents to detect APPL1. ELISA - This antibody can be used at 0.5 - 1.0 μg/mL with the appropriate secondary reagents to detect APPL1. Immunoprecipitation – See reference [6], [11]

Store at –20°C. For long-term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.

1. Hosch, S.E., J.M. Olefsky, and J.J. Kim, APPLied mechanics: uncovering how adiponectin modulates insulin action. Cell Metab, 2006. 4(1): p. 5-6. 2. Mitsuuchi, Y., et al., Identification of a chromosome 3p14.3-21.1 gene, APPL, encoding an adaptor molecule that interacts with the oncoprotein- serine/threonine kinase AKT2. Oncogene., 1999. 18(35): p. 4891-8. 3. Lin, D.C., et al., APPL1 associates with TrkA and GIPC1, and is required for NGF-mediated signal transduction. Mol Cell Biol, 2006. 25: p. 25. 4. Varsano, T., et al., GIPC is recruited by APPL to peripheral TrkA endosomes and regulates TrkA trafficking and signaling. Mol Cell Biol, 2006. 26(23): p. 8942-52. 5. Liu, J., et al., Mediation of the DCC apoptotic signal by DIP13 alpha. J Biol Chem., 2002. 277(29): p. 26281-5. Epub 2002 May 14. 6. Cheng, K.K., et al., Adiponectin-induced endothelial nitric oxide synthase activation and nitric oxide production are mediated by APPL1 in endothelial cells. Diabetes, 2007. 56(5): p. 1387-94. 7. Mao, X., et al., APPL1 binds to adiponectin receptors and mediates adiponectin signalling and function. Nat Cell Biol., 2006. 8(5): p. 516-23. Epub 2006 Apr 16. 8. Nechamen, C.A., et al., Human follicle-stimulating hormone (FSH) receptor interacts with the adaptor protein APPL1 in HEK 293 cells: potential involvement of the PI3K pathway in FSH signaling. Biol Reprod., 2004. 71(2): p. 629-36. Epub 2004 Apr 7. 9. Miaczynska, M., et al., APPL proteins link Rab5 to nuclear signal transduction via an endosomal compartment. Cell., 2004. 116(3): p. 445-56. 10. Erdmann, K.S., et al., A role of the Lowe syndrome protein OCRL in early steps of the endocytic pathway. Dev Cell, 2007. 13(3): p. 377-90. 11. Cheng, K.K., et al., APPL1 potentiates insulin-mediated inhibition of hepatic glucose production and alleviates diabetes via Akt activation in mice. Cell Metab, 2009. 9(5): p. 417-27.

Int J Cardiol 2016 Aug [216: 32-42], Metabolism 2014 [63(10):1363 - 1373]