chemical

7954/10U

7954/10U

10 ug

445 USD

7954/10U

CELT-327

10 ug

Potent and selective hA2B/A3 adenosine receptor fluorescent antagonist

Fluorescent Receptor Probes

Small Molecules

95%

1334.19

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CELT-327 is a potent and selective fluorescent hA2B/A3 adenosine receptor antagonist (Ki values are 35.6 nM and 45.7 nM at A2B and A3 receptors respectively). It shows dose-dependent membrane fluorescent labeling in HCT116 cells expressing A2B adenosine receptors, with rapid diffusion and homogeneous distribution of the fluorescence signal within the cell monolayer. In live HCT116 compact spheroids, CELT-327 displays homogeneous staining across the entire spheroid diameter, reaching deep spheroid regions. CELT-327 can be used as a fluorescent adenosine receptor probe for fluorescence binding assays, live cell imaging, flow cytometry, and fluorescence polarization assays. It is a suitable replacement for GPCR radiolabeling ligands. Excitation and emission maxima ( ) are 589 nm and 616 nm, respectively. Wavelengths are compatible with use as an acceptor dye in TR-FRET assays, together with the CoraFluor 1 (Cat. No. 7920) TR-FRET donor. . Add 75 L of assay buffer containing 1% of DMSO to obtain a 100 M stock solution, and use 250 nM as a working concentration.

Application of CELT-327 to live colorectal cancer cells

Flow Cytometry

Potent and selective hA2B/A3 adenosine receptor fluorescent antagonist

445 USD

Potent and selective hA2B/A3 adenosine receptor fluorescent antagonist

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