chemical

7483/2

7483/2

2 mg

315 USD

7483/2

BDY FL VH032

2 mg

High-affinity VHL fluorescent probe for TR-FRET and FP assays

Fluorescent Probes

Small Molecules

95%

937.91

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BDY FL VH032 is a high-affinity VHL fluorescent probe (Kd = 3.01 nM) with application in time-resolved fluorescence resonance energy-transfer (TR-FRET) assays for high-throughput identification and characterization of VHL ligands. BDY FL VH032 consists of a derivative of von Hippel-Lindau (VHL) ligand VH 032 (Cat. No. 6911) and a fluorophore BDY FL (Cat. No. 5465) (with excitation peak at 504 nm and emission peak at 520 nm), joined by a polyethylene glycol (PEG) 4 linker. The BDY FL VH032 VHL TR-FRET signal is stable through the 90 - 300 min incubation time, and the TR-FRET binding assay is sensitive, selective and resistant to assay interference. BDY FL VH032 also exhibits high VHL affinity in a fluorescence polarization (FP) assay. Excitation maximum ~ 504 nm; emission maximum ~ 520 nm.

High-affinity VHL fluorescent probe for TR-FRET and FP assays

High-affinity VHL fluorescent probe for TR-FRET and FP assays

(2S,4R)-1-((S)-2-(tert-Butyl)-21-(5,5-difluoro-7,9-dimethyl-5H-5 4,6 4-dipyrrolo[1,2-c:2 ,1 -f][1,3,2]diazaborinin-3-yl)-4,19-dioxo-6,9,12,15-tetraoxa-3,18-diazahenicosanoyl)-4-hydroxy-N-(4-(4-methylthiazol-5-yl)benzyl)pyrrolidine-2-ca

C46H62BF2N7O9S

C46H62BF2N7O9S

2770675-66-4

315 USD

High-affinity VHL fluorescent probe for TR-FRET and FP assays

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