chemical

7392/10

7392/10

10 mg (also 50 mg)

148 USD

7392/10

5-Ph-IAA

10 mg (also 50 mg)

Selective and potent TAG Degrader for auxin-inducible degron 2 system

Active Degraders

Small Molecules

98%

251.29

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5-Ph-IAA is a selective and potent Bump Hole TAG Degrader (DC50,6h = 17.0 nM, T1/2 = 62.3 min), for use within an auxin-inducible degron (AID2) system. 5-Ph-IAA is an analog (the Bump ) of natural auxin that induces rapid degradation of a target protein tagged with mAID F-box protein (the Hole or mini AID), and recruits a mutant E3 ligase complex, OsTIR1(F74G). The induced degradation is reversible 3 h after washout with fresh medium. 5-Ph-IAA shows rapid depletion of mAID-tagged nuclear and cytoplasmic proteins in proliferating and non-proliferating mammalian cells with no detectable basal degradation, lower cytotoxicity level, increased potency and faster degradation of the reporter in comparison with the conventional AID system. The AID2 system also works in budding yeast and fission yeast, and it can control protein expression in multiple mouse organs in vivo . . 5-Ph-IAA-AM (Cat. No. 7893), an analog of 5-Ph-IAA, is also available. . Plasmid vectors for the AAV transfection and CRISPR-mediated knock-in of OsTIR1F74G and mAID tag are available from Addgene .

Selective and potent TAG Degrader for auxin-inducible degron 2 system

Selective and potent TAG Degrader for auxin-inducible degron 2 system

5-Phenyl-1H-indole-3-acetic acid

C16H13NO2

C16H13NO2

168649-23-8

148 USD

Selective and potent TAG Degrader for auxin-inducible degron 2 system

Store at -20░C