chemical

Hoechst Janelia Fluor 646

6804/100U

100 ug

361 USD

6804

6804/100U

Hoechst Janelia Fluor 646

Fluorogenic, red-emitting DNA probe. Used for: fixed and live-cell imaging. Application: confocal microscopy, super resolution microscopy (SRM) (PALM, PAINT), lattice light sheet microscopy

Fluorescent DNA Stains

100 ug

Small Molecules

90%

Store at -20°C

1119.39

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Key information: Hoechst Janelia Fluor 646 is a fluorogenic red-emitting DNA probe. Suitable for fixed and live-cell imaging. . Application :confocal microscopy, super resolution microscopy (SRM) (including PALM and PAINT), lattice light sheet microscopy. . Properties and Photophysical Data : Hoechst Janelia Fluor 646 preferentially stains and binds minor groove of AT-rich regions. The compound can be combined with fluorogenic green-emitting DNA probe Hoechst Janelia Fluor 526 (Cat. No. 7313) for multiplexing experiments. Hoechst Janelia Fluor 646 can also be combined with Hoechst Janelia Fluor 526 to perform dual-color STED using the same depletion laser ( dep = 775 nm). Sub-diffraction spatial resolution STED imaging can be achieved using Hoechst Janelia Fluor 646 in E. coli cells (~100 nM - 1 M). Hoechst Janelia Fluor 646 is a desirable alternative to large oligonucleotide-conjugated antibodies for PAINT experiments, particularly for bacterial studies. Excitation and emission maxima ( ) are 655 nm and 670 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1. . Hoechst Janelia Fluor 646 is fluorogenic and fluoresces only once bound to DNA, enabling hassle-free no-wash experiments. . To measure the absorbance spectrum of Hoechst Janelia Fluo 646 we recommend the following solvent: TFE plus 0.1% TFA.

Flow Cytometry, Immunohistochemistry, Immunocytochemistry

JF646-Hoechst Hoechst-JF646 Hoechst Janelia Fluor646 fluorogenic red emitting DNA probes stains dyes live cell imaging ICC IHC flow cytometry Hoechst JF 646 JF646-Hoechst Fluorescent DNA Stains Flow Cytometry Counterstains 6804,

Fluorogenic, red-emitting DNA probe. Used for: fixed and live-cell imaging. Application: confocal microscopy, super resolution microscopy (SRM) (PALM, PAINT), lattice light sheet microscopy

2-(3-(Azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-((2-(2-(2-(4-(4-(5-(4-methylpiperazin-1-yl)-1H,3 H-[2,5 -bibenzo[d]imidazol]-2 -yl)phenoxy)butanamido)ethoxy)ethoxy)ethyl)carbamoyl)benzoat

C64H70N10O7Si

C64H70N10O7Si

2462011-60-3

346

361 USD

Fluorogenic, red-emitting DNA probe. Used for: fixed and live-cell imaging. Application: confocal microscopy, super resolution microscopy (SRM) (PALM, PAINT), lattice light sheet microscopy

Hoechst JF 646,JF646-Hoechst