dTAG-13 | Tocris Bioscience 6605/5 product information

product summary

company name :

Tocris Bioscience

product type :

chemical

product name :

dTAG-13

catalog :

6605/5

quantity :

5 mg

price :

540 USD

more info or order :

Tocris Bioscience product webpage

citations: 20

Reference
Nirala B, Patel T, Kurenbekova L, Shuck R, Dasgupta A, Rainusso N, et al. MYC regulates CSF1 expression via microRNA 17/20a to modulate tumor-associated macrophages in osteosarcoma. JCI Insight. 2023;8: pubmed publisher
Noviello G, Gjaltema R, Schulz E. CasTuner is a degron and CRISPR/Cas-based toolkit for analog tuning of endogenous gene expression. Nat Commun. 2023;14:3225 pubmed publisher
Orth He E, Huang H, Rao S, Wang Q, Chen Q, O Mara C, et al. Protein folding stress potentiates NLRP1 and CARD8 inflammasome activation. Cell Rep. 2023;42:111965 pubmed publisher
Ma D, Yuan Q, Peng F, Paredes V, Zeng H, Osikpa E, et al. Engineered PROTAC-CID Systems for Mammalian Inducible Gene Regulation. J Am Chem Soc. 2023;: pubmed publisher
Yu D, Chen G, Wang Y, Wang Y, Lin R, Liu N, et al. Regulation of cohesin-mediated chromosome folding by PDS5 in mammals. EMBO Rep. 2022;23:e54853 pubmed publisher
Bondeson D, Mullin Bernstein Z, Oliver S, Skipper T, Atack T, Bick N, et al. Systematic profiling of conditional degron tag technologies for target validation studies. Nat Commun. 2022;13:5495 pubmed publisher
Abuhashem A, Hadjantonakis A. Generation of knock-in degron tags for endogenous proteins in mice using the dTAG system. STAR Protoc. 2022;3:101660 pubmed publisher
Molenaar T, Malik M, Silva J, Liu N, Haarhuis J, Ambrosi C, et al. The histone methyltransferase SETD2 negatively regulates cell size. J Cell Sci. 2022;135: pubmed publisher
Huang X, Bashkenova N, Hong Y, Lyu C, Guallar D, Hu Z, et al. A TET1-PSPC1-Neat1 molecular axis modulates PRC2 functions in controlling stem cell bivalency. Cell Rep. 2022;39:110928 pubmed publisher
Bowness J, Nesterova T, Wei G, Rodermund L, Almeida M, Coker H, et al. Xist-mediated silencing requires additive functions of SPEN and Polycomb together with differentiation-dependent recruitment of SmcHD1. Cell Rep. 2022;39:110830 pubmed publisher
Hsiao J, Neugroschl A, Chui A, Taabazuing C, Griswold A, Wang Q, et al. A ubiquitin-independent proteasome pathway controls activation of the CARD8 inflammasome. J Biol Chem. 2022;298:102032 pubmed publisher
Bao H, Carraro M, Flury V, Liu Y, Luo M, Chen L, et al. NASP maintains histone H3-H4 homeostasis through two distinct H3 binding modes. Nucleic Acids Res. 2022;50:5349-5368 pubmed publisher
Xie L, Dong P, Qi Y, Hsieh T, English B, Jung S, et al. BRD2 compartmentalizes the accessible genome. Nat Genet. 2022;54:481-491 pubmed publisher
Fiskus W, Boettcher S, Daver N, Mill C, Sasaki K, Birdwell C, et al. Effective Menin inhibitor-based combinations against AML with MLL rearrangement or NPM1 mutation (NPM1c). Blood Cancer J. 2022;12:5 pubmed publisher
Guarnaccia A, Weissmiller A, Tansey W. Gene-specific quantification of nascent transcription following targeted degradation of endogenous proteins in cultured cells. STAR Protoc. 2021;2:101000 pubmed publisher
Sahillioğlu A, Toebes M, Apriamashvili G, Gomez R, Schumacher T. CRASH-IT Switch Enables Reversible and Dose-Dependent Control of TCR and CAR T-cell Function. Cancer Immunol Res. 2021;9:999-1007 pubmed publisher
Damhofer H, Radzisheuskaya A, Helin K. Generation of locus-specific degradable tag knock-ins in mouse and human cell lines. STAR Protoc. 2021;2:100575 pubmed publisher
Sharif H, Hollingsworth L, Griswold A, Hsiao J, Wang Q, Bachovchin D, et al. Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome formation by sequestering its active C-terminal fragment. Immunity. 2021;54:1392-1404.e10 pubmed publisher
Kaji T, Koga H, Kuroha M, Akimoto T, Hayata K. Characterization of cereblon-dependent targeted protein degrader by visualizing the spatiotemporal ternary complex formation in cells. Sci Rep. 2020;10:3088 pubmed publisher
Li S, Prasanna X, Salo V, Vattulainen I, Ikonen E. An efficient auxin-inducible degron system with low basal degradation in human cells. Nat Methods. 2019;16:866-869 pubmed publisher

product information

brand :

Tocris

MasterCode :

6605

SKU :

6605/5

product name :

dTAG-13

target :

TAG Degradation Platforms

category :

Small Molecules

unit size :

5 mg

purity :

98%

observed molecular weight :

1049.18

url print :

?utm_source=biocompare&utm_medium=referral&utm_campaign=product&utm_term=smallmolecules

details of functionality :

dTAG-13 is a degrader targeting mutant FKBP12F36V fusion proteins. Comprises a ligand selective for F36V single-point mutated FKBP12, a linker and a cereblon-binding ligand. Application of dTAG-13 induces rapid, reversible and selective degradation of FKBP12F36V fusion proteins in vitro and in vivo . dTAG is generalizable to a range of fusion proteins; useful as an alternative to genetic methods for target validation. . Negative control (Cat. No. 6916) also available. . FKBP12F36V can be expressed as a fusion with a target protein of interest using genome engineering techniques, via transgene expression or CRISPR-mediated locus-specific knock-in. Custom knock-in cell lines for the dTAG and aTAG platforms are available from our sister brand R D Systems . Email TPD@bio-techne.com to enquire. . Plasmid vectors for the lentiviral expression and CRISPR-mediated knock-in of FKBP12F36V are available from Addgene.

extended description :

Degrades mutant FKBP12F36V fusion proteins; useful alternative to genetic methods for target validation

chemical name text :

1-[(2S)-1-Oxo-2-(3,4,5-trimethoxyphenyl)butyl]-(2S)-2-piperidinecarboxylate (1R)-3-(3,4-dimethoxyphenyl)-1-[2-[2-[[6-[[2-(2,6-dioxo-3-piperidinyl)-2,3-dihydro-1,3-dioxo-1H-isoindol-4-yl]oxy]hexyl]amino]-2-oxoethoxy]phenyl]propyl ester

formula :

C 57 H 68 N 4 O 15

formula text :

C57H68N4O15

cas num :

2064175-41-1

USD :

540 USD

product details :

Degrades mutant FKBP12F36V fusion proteins; useful alternative to genetic methods for target validation

storage :

Store at -20°C

more info or order :

Tocris Bioscience product webpage