chemical

6419/2

6419/2

2 mg

475 USD

6419/2

Janelia Fluor 635, NHS ester

2 mg

Red dye (Reactive group: NHS ester). Application: flow cytometry, confocal microscopy, super resolution microscopy. Suitable for live cell imaging

Amine-Reactive Janelia Fluor Dyes

Small Molecules

95%

629.69

?utm_source=labome&utm_medium=referral&utm_campaign=product&utm_term=smallmolecules

Key Information: Janelia Fluor 635, NHS ester is a red fluorogenic fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live cell imaging. . Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). Cell permeable. . Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag and SNAP-tag . Excitation and emission maxima ( ) are 635 nm and 652 nm, respectively; quantum yield = 0.56; extinction coefficient = 167,000 M-1cm-1 (measured in ethanol or TFE plus 0.1% TFA); A280 correction factor = 0.0524; lactone-zwitterion equilibrium constant (KL-Z) 0.0001. Exhibits low nonspecific background staining. . Please see the protocol for further information on protein/antibody labeling and conjugation. . To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA. . We also offer Janelia Fluor conjugated antibodies and custom conjugation services . . HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc. . Janelia Fluor is a registered trademark of Howard Hughes Medical Institute. . This product has been recently renamed. The previous name for this product was Janelia Fluor 635, SE.

Applications on Mitochondria for Janelia Fluor« 635, NHS ester

Immunohistochemistry, Immunocytochemistry

Red dye (Reactive group: NHS ester). Application: flow cytometry, confocal microscopy, super resolution microscopy. Suitable for live cell imaging

1-[10-[2-Carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-9,9-dimethyl-7-[1-(3-fluoroazetidinyl)]-9-silaanthracen-2(9H)-ylidene]-3-fluoroazetidinium, inner salt

C33H29F2N3O6Si

C33H29F2N3O6Si

475 USD

Red dye (Reactive group: NHS ester). Application: flow cytometry, confocal microscopy, super resolution microscopy. Suitable for live cell imaging

JF 635,NHS

Store at -20░C