product summary
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company name :
Tocris Bioscience
product type :
chemical
product name :
6418/1
catalog :
6418/1
quantity :
1 mg
price :
329 USD
more info or order :
image
image 1 :
Tocris Bioscience 6418/1 image 1
Orange dye (Reactive group: NHS ester). Applications: super resolution microscopy, confocal, two-photon excitation and live cell imaging
product information
master code :
6418
SKU :
6418/1
product name :
Janelia Fluor 585, NHS ester
unit size :
1 mg
description :
Orange dye (Reactive group: NHS ester). Application: confocal microscopy, super resolution microscopy, and two-photon excitation fluorescence microscopy. Suitable for live cell imaging
target :
Amine-Reactive Janelia Fluor Dyes
category :
Small Molecules
purity :
95%
observed molecular weight :
649.6
url print :
?utm_source=labome&utm_medium=referral&utm_campaign=product&utm_term=smallmolecules
details of functionality :
Key Information: Janelia Fluor 585, NHS ester is an orange fluorogenic fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live cell imaging. . Applications: Suitable for confocal microscopy, two-photon excitation fluorescence microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). Cell permeable. . Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag and SNAP-tag . Excitation and emission maxima ( ) are 585 nm and 609 nm, respectively; quantum yield = 0.78; extinction coefficient = 156,000 M-1cm-1 (measured in Ethanol or TFE plus 0.1% TFA); A280 correction factor = 0.099; lactone-zwitterion equilibrium constant (KL-Z) 0.0001. Exhibits low non-specific background staining. . Please see the protocol for further information on protein/antibody labeling and conjugation. . To measure the absorbance spectrum of this dye we recommend the following solvent: trifluoroethanol plus 0.1% TFA. . We also offer Janelia Fluor conjugated antibodies and custom conjugation services . . HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc. . Janelia Fluor is a registered trademark of Howard Hughes Medical Institute. . This product has been recently renamed. The previous name for this product was Janelia Fluor 585, SE.
top caption :
Orange dye (Reactive group: NHS ester). Applications: super resolution microscopy, confocal, two-photon excitation and live cell imaging
applications :
Immunohistochemistry, Immunocytochemistry
extended description :
Orange dye (Reactive group: NHS ester). Application: confocal microscopy, super resolution microscopy, and two-photon excitation fluorescence microscopy. Suitable for live cell imaging
chemical name text :
1-[10-[2-Carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-7-(3,3-difluoro-1-azetidinyl)-9,9-dimethyl-2(9H)-anthracenylidene]-3,3-difluoroazetidinium, inner salt
formula :
C34H27F4N3O6
formula text :
C34H27F4N3O6
cas num :
1811539-88-4
USD :
329 USD
product details :
Orange dye (Reactive group: NHS ester). Application: confocal microscopy, super resolution microscopy, and two-photon excitation fluorescence microscopy. Suitable for live cell imaging
alt names :
JF 585,NHS
storage :
Store at -20░C
more info or order :
company information
Tocris Bioscience
The Watkins Building
Atlantic Road
Avonmouth, Bristol
BS11 9QD
info@bio-techne.com
https://www.tocris.com
800-343-7475
headquarters: UK