product summary
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company name :
Tocris Bioscience
product type :
chemical
product name :
PA Janelia Fluor 549, SE
catalog :
6149/300U
quantity :
300 ug
price :
172 USD
more info or order :
citations: 1
Reference
Genshaft A, Ziegler C, Tzouanas C, Mead B, Jaeger A, Navia A, et al. Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes. Nat Commun. 2021;12:4995 pubmed publisher
product information
brand :
Tocris
MasterCode :
6149
SKU :
6149/300U
product name :
PA Janelia Fluor 549, SE
target :
Photoactivatable Dyes
category :
Small Molecules
unit size :
300 ug
purity :
90%
observed molecular weight :
575.58
url print :
?utm_source=biocompare&utm_medium=referral&utm_campaign=product&utm_term=smallmolecules
details of functionality :
PA Janelia Fluor 549, SE is a photoactivatable fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. Non-fluorescent until activated at 365 nm. Photochemical quantum yield of uncaging = 2.2%, with improved yield upon protein conjugation. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag and SNAP-tag . Suitable for single molecule tracking and super resolution microscopy in live cells, specifically live cell sptPALM and fixed cell PALM. Can be multiplexed with PA Janelia Fluor 646, SE (Cat. No. 6150) to perform two-color sptPALM in live cells with activation at 405 nm. Cell permeable. Excitation maximum = 551 - 553 nm; emission maximum = 570 - 573 nm. . Please see the protocol for further information on protein/antibody labeling and conjugation. . HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
extended description :
Yellow photoactivatable dye (Reactive group: NHS ester). Applications: super resolution microscopy (PALM), single molecule tracking and live cell imaging
chemical name text :
2,5-Dioxo-1-pyrrolidinyl 3 ,6 -di-1-azetidinyl-2-diazo-2,3-dihydro-3-oxospiro[1H-indene-1,9 -[9H]xanthene]-6-carboxylate
formula :
C 32 H 25 N 5 O 6
formula text :
C32H25N5O6
cas num :
1811539-42-0
USD :
172 USD
product details :
Yellow photoactivatable dye (Reactive group: NHS ester). Applications: super resolution microscopy (PALM), single molecule tracking and live cell imaging
storage :
Store at -20°C
more info or order :
company information
Tocris Bioscience
The Watkins Building
Atlantic Road
Avonmouth, Bristol
BS11 9QD
info@bio-techne.com
https://www.tocris.com
800-343-7475
headquarters: UK