chemical

Janelia Fluor 646, SE

6148

1 mg

239 USD

6148

6148/1

Janelia Fluor 646, SE

Red dye (Reactive group: NHS ester). Applications: super resolution microscopy e.g. dSTORM STED, confocal, flow and live cell imaging

Amine-Reactive Janelia Fluor® Dyes

Small Molecules

1 mg

593.71

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Fluorogenic fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag and SNAP-tag . Suitable for confocal fluorescent imaging, super resolution microscopy (SRM) techniques such as dSTORM (live and fixed cells) and STED imaging. Can be multiplexed for two colour imaging with Janelia Fluor 549 SE (Cat. No. 6147). Also suitable for flow cytometry. Cell permeable. Excitation maximum = 646 nm; emission maximum = 664 nm. Quantum yield = 0.54, Max. extinction coefficient = 152,000 M-1cm-1 (measured in ethanol plus 0.1% TFA); A280 correction factor is 0.19. . We recommend that stock solutions of this dye are prepared in anhydrous DMF. . To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA. . We also offer Janelia Fluor conjugated antibodies and custom conjugation services with our sister company Novus Biologicals. . HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

6148

JF646 Janelia Fluor 646-SE fluorescent dyes NHS ester self-labeling tag systems microscopy cell permeable super resolution STED flow cytometry JF 646, NHS Amine Reactive Janelia Fluor Dyes Flow Cytometry 6148, Janelia Fluor, fluorescent dye, amine-reactive

Red dye (Reactive group: NHS ester). Applications: super resolution microscopy e.g. dSTORM STED, confocal, flow and live cell imaging

1-[7-(1-Azetidinyl)-10-[2-carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-9,9-dimethyl-9-silaanthracen-2(9H)-ylidene]azetidinium, inner salt

C 33 H 31 N 3 O 6 Si

C33H31N3O6Si

1811539-59-9

239

239 USD

JF 646, NHS

Store at -20°C