product summary
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company name :
Tocris Bioscience
product type :
chemical
product name :
Janelia Fluor 646, SE
catalog :
6148/1
quantity :
1 mg
price :
297 USD
more info or order :
citations: 5
Reference
Gonschior H, Schmied C, Van der Veen R, Eichhorst J, Himmerkus N, Piontek J, et al. Nanoscale segregation of channel and barrier claudins enables paracellular ion flux. Nat Commun. 2022;13:4985 pubmed publisher
Kanagy W, Cleyrat C, Fazel M, Lucero S, Bruchez M, Lidke K, et al. Docking of Syk to FcεRI is enhanced by Lyn but limited in duration by SHIP1. Mol Biol Cell. 2022;:mbcE21120603 pubmed publisher
Saric A, Freeman S, Williamson C, Jarnik M, Guardia C, Fernandopulle M, et al. SNX19 restricts endolysosome motility through contacts with the endoplasmic reticulum. Nat Commun. 2021;12:4552 pubmed publisher
Mei L, Espinosa de Los Reyes S, Reynolds M, Leicher R, Liu S, Alushin G. Molecular mechanism for direct actin force-sensing by α-catenin. elife. 2020;9: pubmed publisher
Mazloom Farsibaf H, Kanagy W, Lidke D, Lidke K. High-speed single molecule imaging datasets of membrane proteins in rat basophilic leukemia cells. Data Brief. 2020;30:105424 pubmed publisher
product information
brand :
Tocris
MasterCode :
6148
SKU :
6148/1
product name :
Janelia Fluor 646, SE
target :
Amine-Reactive Janelia Fluor Dyes
category :
Small Molecules
unit size :
1 mg
observed molecular weight :
593.71
url print :
?utm_source=biocompare&utm_medium=referral&utm_campaign=product&utm_term=smallmolecules
details of functionality :
Janelia Fluor 646, SE is a fluorogenic fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag and SNAP-tag . Suitable for confocal fluorescent imaging, super resolution microscopy (SRM) techniques such as dSTORM (live and fixed cells) and STED imaging. Can be multiplexed for two colour imaging with Janelia Fluor 549 SE (Cat. No. 6147). Also suitable for flow cytometry. Cell permeable. Excitation maximum = 646 nm; emission maximum = 664 nm. Quantum yield = 0.54, Max. extinction coefficient = 152,000 M-1cm-1 (measured in TFE plus 0.1% TFA); A280 correction factor is 0.19. . Please see the protocol for further information on protein/antibody labeling and conjugation. . We recommend that stock solutions of this dye are prepared in anhydrous DMF. . To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA. . We also offer Janelia Fluor conjugated antibodies and custom conjugation services with our sister company Novus Biologicals. . HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
applications :
Flow Cytometry, Immunohistochemistry, Immunocytochemistry
extended description :
Red dye (Reactive group: NHS ester). Applications: super resolution microscopy e.g. dSTORM STED, confocal, flow and live cell imaging
chemical name text :
1-[7-(1-Azetidinyl)-10-[2-carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-9,9-dimethyl-9-silaanthracen-2(9H)-ylidene]azetidinium, inner salt
formula :
C 33 H 31 N 3 O 6 Si
formula text :
C33H31N3O6Si
cas num :
1811539-59-9
USD :
297 USD
product details :
Red dye (Reactive group: NHS ester). Applications: super resolution microscopy e.g. dSTORM STED, confocal, flow and live cell imaging
alt names :
JF 646, NHS
storage :
Store at -20°C
more info or order :
company information
Tocris Bioscience
The Watkins Building
Atlantic Road
Avonmouth, Bristol
BS11 9QD
info@bio-techne.com
https://www.tocris.com
800-343-7475
headquarters: UK