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company name :
Tocris Bioscience
product type :
chemical
product name :
CHIR 99021
catalog :
4423/10
quantity :
10 mg (also 50 mg)
price :
265 USD
more info or order :
citations: 115
Reference
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Schneider O, Zeifang L, Fuchs S, Sailer C, Loskill P. User-friendly & parallelized generation of hiPSC-derived µ-tissues in a centrifugal Heart-on-a-Chip. Tissue Eng Part A. 2019;: pubmed publisher
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Sharma A, Zhang Y, Buikema J, Serpooshan V, Chirikian O, Kosaric N, et al. Stage-specific Effects of Bioactive Lipids on Human iPSC Cardiac Differentiation and Cardiomyocyte Proliferation. Sci Rep. 2018;8:6618 pubmed publisher
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Bantounas I, Ranjzad P, Tengku F, Silajdzic E, Forster D, Asselin M, et al. Generation of Functioning Nephrons by Implanting Human Pluripotent Stem Cell-Derived Kidney Progenitors. Stem Cell Reports. 2018;10:766-779 pubmed publisher
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Vermilyea S, Guthrie S, Meyer M, Smuga Otto K, Braun K, Howden S, et al. Induced Pluripotent Stem Cell-Derived Dopaminergic Neurons from Adult Common Marmoset Fibroblasts. Stem Cells Dev. 2017;26:1225-1235 pubmed publisher
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McCauley K, Hawkins F, Serra M, Thomas D, JACOB A, Kotton D. Efficient Derivation of Functional Human Airway Epithelium from Pluripotent Stem Cells via Temporal Regulation of Wnt Signaling. Cell Stem Cell. 2017;20:844-857.e6 pubmed publisher
Lai F, Lau S, Wong J, Gui H, Wang R, Zhou T, et al. Correction of Hirschsprung-Associated Mutations in Human Induced Pluripotent Stem Cells Via Clustered Regularly Interspaced Short Palindromic Repeats/Cas9, Restores Neural Crest Cell Function. Gastroenterology. 2017;153:139-153.e8 pubmed publisher
Sampaziotis F, de Brito M, Geti I, Bertero A, Hannan N, Vallier L. Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells. Nat Protoc. 2017;12:814-827 pubmed publisher
Yang T, Chen Q, Deng J, Jagannathan G, Tobias J, Schultz D, et al. Mutual reinforcement between telomere capping and canonical Wnt signalling in the intestinal stem cell niche. Nat Commun. 2017;8:14766 pubmed publisher
Tang Y, Cheng L. Cocktail of chemical compounds robustly promoting cell reprogramming protects liver against acute injury. Protein Cell. 2017;8:273-283 pubmed publisher
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Chal J, Al Tanoury Z, Hestin M, Gobert B, Aivio S, Hick A, et al. Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro. Nat Protoc. 2016;11:1833-50 pubmed publisher
Takasato M, Er P, Chiu H, Little M. Generation of kidney organoids from human pluripotent stem cells. Nat Protoc. 2016;11:1681-92 pubmed publisher
Woo D, Chen Q, Yang T, Glineburg M, Hoge C, Leu N, et al. Enhancing a Wnt-Telomere Feedback Loop Restores Intestinal Stem Cell Function in a Human Organotypic Model of Dyskeratosis Congenita. Cell Stem Cell. 2016;19:397-405 pubmed publisher
Mou H, Vinarsky V, Tata P, Brazauskas K, Choi S, Crooke A, et al. Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal Cells. Cell Stem Cell. 2016;19:217-231 pubmed publisher
Capowski E, Wright L, Liang K, Phillips M, Wallace K, Petelinsek A, et al. Regulation of WNT Signaling by VSX2 During Optic Vesicle Patterning in Human Induced Pluripotent Stem Cells. Stem Cells. 2016;34:2625-2634 pubmed publisher
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Leung A, Murdoch B, Salem A, Prasad M, Gomez G, García Castro M. WNT/β-catenin signaling mediates human neural crest induction via a pre-neural border intermediate. Development. 2016;143:398-410 pubmed publisher
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Sladitschek H, Neveu P. The bimodally expressed microRNA miR-142 gates exit from pluripotency. Mol Syst Biol. 2015;11:850 pubmed publisher
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Zhang L, Yin J, Yeh H, Ma N, Lee G, Chen X, et al. Small Molecules Efficiently Reprogram Human Astroglial Cells into Functional Neurons. Cell Stem Cell. 2015;17:735-47 pubmed publisher
Morizane R, Lam A, Freedman B, Kishi S, Valerius M, Bonventre J. Nephron organoids derived from human pluripotent stem cells model kidney development and injury. Nat Biotechnol. 2015;33:1193-200 pubmed
Hugo W, Shi H, Sun L, Piva M, Song C, Kong X, et al. Non-genomic and Immune Evolution of Melanoma Acquiring MAPKi Resistance. Cell. 2015;162:1271-85 pubmed publisher
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product information
brand :
Tocris
master code :
4423
SKU :
4423/10
rank :
0.081
product name :
CHIR 99021
description :
Highly selective GSK-3 inhibitor; acts as Wnt activator
target :
GSK-3 Inhibitors
category :
Small Molecules
sizes available :
10 mg (also 50 mg)
url print :
?utm_source=biocompare&utm_medium=referral&utm_campaign=product&utm_term=smallmolecules
details of functionality :
CHIR 99021 is a potent and highly selective inhibitor of glycogen synthase kinase 3 (GSK-3) (IC50 values are 6.7 and 10 nM for GSK-3 and GSK-3 respectively). Acts as Wnt activator. Exhibits 500-fold selectivity for GSK-3 over closely related kinases; also displays 800-fold selectivity against 45 additional enzymes and receptors. In combination with the irreversible inhibitor of LSD1 (Cat. No. 3852), CHIR 99021 enables reprogramming of mouse embryonic fibroblasts, transduced by Oct4 and Klf4 only, into iPSCs. Enhances mouse and human ESC self-renewal when used in combination with PD 0325901 (Cat. No. 4192). It promotes differentiation of hPSCs in cardiomyocytes and allows formation of extended pluripotent stem (EPS) cells in combination with (S)-(+)-Dimethindene maleate (Cat. No. 1425), tetracycline antibiotic (Cat. No. 3268) and human leukemia inhibitory factor. . CHIR 99021 synthesized to cGMP guidelines also available. . For more information on how to use CHIR 99021, see our protocols: Transdifferentiating Fibroblasts into Neurons, Culturing Transcriptomically Distinct Pluripotent mESCs, Converting Fibroblasts into Cardiomyocytes (9C Cocktail), Human iPSC-derived Myoblasts .
extended description :
Highly selective GSK-3 inhibitor; acts as Wnt activator
chemical name text :
6-[[2-[[4-(2,4-Dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)-2-pyrimidinyl]amino]ethyl]amino]-3-pyridinecarbonitrile
formula :
C 22 H 18 Cl 2 N 8
formula text :
C22H18Cl2N8
cas num :
252917-06-9
USD :
265 USD
product details :
Highly selective GSK-3 inhibitor; acts as Wnt activator
alt names :
CHIR99021, CT99021
storage :
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
more info or order :
company information
Tocris Bioscience
The Watkins Building
Atlantic Road
Avonmouth, Bristol
BS11 9QD
info@bio-techne.com
https://www.tocris.com
800-343-7475
headquarters: UK